Fluorescence in situ hybridization and genomic in situ hybridization to identify the parental genomes in the intergeneric hybrid between Chrysanthemum japonicum and Nipponanthemum nipponicum
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概要
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Chrysanthemum japonicum (2n=18; ?? ) and Nipponanthemum nipponicum (2n=18; ?? ) were artificially crossed and produced healthy F1 hybrid plants by aids of the series of embryo rescue, axenic culture and acclimatization. FISH biotinlabeled probes of 5S rDNA and pTa71 for 45S rDNA distinguished 5S rDNA and NOR regions of the chromosomes in the genome of respective species in the chromosome complement of the F1 hybrid. One 5S rDNA-signal and two 45S rDNA-signals were shown in the nine chromosomes of C. japonicum genome in the F1 hybrid chromosome complement (2n=18), while no 5S rDNA-signal and one 45S rDNA-signal was shown in the nine chromosomes of N. nipponicum genome. GISH differentiated the genome of C. japonicum and that of N. nipponicum in the F1 hybrid chromosome complement using the probe of total genomic DNA of N. nipponicum and showed that the parental genomes in the chromosome complement of the hybrid were generally in separate domains during mitosis.
著者
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Kondo Katsuhiko
Laboratory Of Plant Chromosome And Gene Stock Faculty Of Science Hiroshima University
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Abd El-twab
Laboratory Of Plant Chromosome And Gene Stock Faculty Of Science Hiroshima University
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