反芻胃の消化に関する研究 : (第2報) Infusoriaの人工培養に就て(その1)

概要

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Numerous protozoaexist in the ruminant, belonging to ciliata and are moving in an active state. In order to cultivate the ciliata artificially, several experiments were undertaken and results obtained are as follows. The rumen ciliata (Infusoria) was obtained through a permanent fistula from goats rumen with its content and concentrated by gentle centrifugation after straining of the content throu adouble surgical gauze. Species of Entodinium were the most among the rumen ciliata of the goat, so we have treated these species mainly, as the subject of cultivation. (1) Infusoria can be alive in vitro for 100_??_120 hrs. in the rumen liquor requiring the coexistence of bacteria. (2) The rumen liquor can be replaced dy the hay extract medium, which is prepared by extracting the hay with RINGERs solution and then incubated with the bacteria of goats rumen for 3 days. (3) There is one necessary factor for the cultivation of Iufusoria in the rumen liquor and this is produced in the hay extract medium by the rumen bacteria after about 3 days incubation. (4) The effectiveness of the factor may be almost !entirely destroyed by heating at 60° for 10 minutes. (5) The survival time of Infusoria varies with the species of bacteria in the culture medium.
社団法人日本農芸化学会の論文