反芻胃の消化に関する研究 : (第3報) Infusoriaの人工培養に就て(其の2)

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Following the previous report, the experiments on the artificial culture of Infusoria from goats rumen have been undertaken. The life span of Infusoria including Entodinia mainly, studied here, can be extended for about twenty days in vitro by addition of fresh white clover leaves at an amount of 5_??_6%; to the hay-RINGERS extract culture medium. which is inoculated with rumen bacteria and incubated for 3 days. It is observed that Infusoria are inan actively motile state and have frequent fission. Infusoria can not survive at various concentrations of the clover juice in the absence of the other essential factors. These effects of fresh white clover leaves on Infusoria cultures are not due to their inorganic substances. When the fresh clover leaves are heated at 100° for 30 minutes, they do not forfeit their properties. So, it may be assumed that the effetcive factor of the clover leaves is a thermostable one. The same effective factor also exists in green peas and spinach leaves.
社団法人日本農芸化学会の論文