カツオ赤血球中の緑色色素に関する研究-I : 単璃精製と二・三の性状

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A unique green pigment occurs in the erythrocytes of scombroid fishes. That of skipjack was separated into six components when applied to Sephadex G-25 (fine) gel filtration. The three major components were further purified by DEAE-cellulose column chromatography and starch block electrophoresis. All the purified components were found to be homogeneous both by disc electrophoresis and isoelectrofocussing. The molecular weights of these components were comparatively small, ranging from 4, 000 to 10, 000. Their isoelectric points were found to be between pH 3.3 and 3.7. The absorption spectra suggested the presence of some nucleotide base(s) as molecular components in addition to the biliverdin-like chromophore, lipid, peptide, sugar, and phosphate moieties thus far reported.
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