サバ科魚類赤血球中における緑色色素の出現ならびに二・三の性状

概要

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A green pigment was found in the lyzates of erythrocytes from scombroid fishes such as mackerel, skipjack, albacore, etc. It migrated much faster than hemoglobin in starch block electrophoresis at pH 8.6. On Sephadex G-25 gel filtration, the pigment separated into three to six components depending upon the species of fish. The colors of those com-ponents generally were slightly different from each other. The green pigment to hemo-globin ratios were estimated to be 1/100 or less in the erythrocytes of these fishes. The green pigment was soluble in water and acetic acid, but not in any of the other organic solvents tested. The aq. solution of the pigment exhibited a characteristic absorp-tion spectrum with a prominent maximum at 370-400 mμ and a rather broad one at 600-650 mμ, suggesting the presence of biliverdin or a related substance as the chromophore. Qualitative examination showed that the pigment is a complex substance consisting of lipid, peptide, sugar, and phosphate in addition to the chromophore.
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