Selective Assay Method with Use of Heat Stability for a Trypsin-sensitive Cholecystokinin (CCK)-releasing Peptide (Monitor Peptide) in Rat Bile-pancreatic Juice(Biological Chemistry)

スポンサーリンク

概要

• 論文の詳細を見る

• A simple and selective assay method for a trypsin-sensitive cholecystokinin (CCK)-releasing peptide ('monitor peptide') found in rat bile-pancreatic juice is reported. This peptide also has trypsin inhibitory activity which could be used as an index for measurement, except that it was difficult to assay the inhibitory activity of the peptide directly in the bile-pancreatic juice because of abundant endogenous trypsin. The peptide and trypsin were purified from rat bile-pancreatic juice, and their sensitivities to heat treatment was examined. Trypsin(ogen) was completely eliminated by being heated at 80℃ for 40 min in the presence of 0.5 M NaCl, pH 2.5, whereas the trypsin inhibitory activity of the peptide was above 95% of the original level after this treatment. Using the difference, we established assay conditions for trypsin inhibition by the peptide in which the effects of endogenous trypsin were removed by heating. This assay method was then used to show that there was a close relationship between trypsin(ogen) and peptide in the bile-pancreatic juice. We estimated that the trypsin inhibitory activity of the peptide masks 2〜5% of the trypsin activity in the bile-pancreatic juice from Wistar male rats fed a stock diet. The optimum conditions for activation of trypsinogen and chymotrypsinogen in rat bile-pancreatic juice were also studied. At 37℃, trypsinogen and chymotrypsinogen were maximally activated by enterokinase 5% of protein being activated for 40 and 6 min, respectively.

• 社団法人日本農芸化学会の論文
• 1987-04-23

著者

• Fushiki Tohru

  Laboratory Of Nutrition Chemistry Division Of Food Science And Biotechnology Graduate School Of Agri

• Fushiki Tohru

  京都大学 農学研究科栄養化学分野

• Iwai K

  Kyoto Univ. Kyoto

• Iwai K

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Iwai Kazuo

  Laboratory Of Nutrition Chemistry Faculty Of Home Economics Kobe Women's University

• Fushiki T

  Laboratory Of Nutrition Chemistry Division Of Food Science And Biotechnology Graduate School Of Agri

• Takada M

  Kyoto Univ. Kyoto Jpn

• Fukuoka Shin-ichi

  Laboratory Of Molecular Cell Biology Department Of Chemistry And Biological Science College Of Scien

• FUKUOKA Shin-ichi

  Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

• TSUJIKAWA Masahiro

  Laboratory of Nutritional Chemistry, Department of Food Science and Technology, Faculty of Agricultu

• Fushiki Tohru

  Laboratory Of Nutrition Chemistry Division Of Applied Life Sciences Graduate School Of Agriculture K

• Fukuoka Shin-ichi

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

関連論文

• Capsinoids, non-pungent capsaicin analogs, reduce body fat accumulation without weight rebound unlike dietary restriction in mice
• Capsinoids, Non-Pungent Capsaicin Analogs, Reduce Body Fat Accumulation without Weight Rebound unlike Dietary Restriction in Mice
• Germinated Barley Foodstuff Improves Constipation Induced by Loperamide in Rats
• Effects of Germinated Barley Foodstuff in Preventing Diarrhea and Forming Normal Feces in Ceco-colectomized Rats
• Preventive Effect of Germinated Barley Foodstuff on Diarrhea Induced by Water-soluble Dietary Fiber in Rats
• Increased Noradrenergic Activity in the Ventromedial Hypothalamus during Treadmill Running in Rats
• Matriptase Does Not Require Hepatocyte Growth Factor Activator Inhibitor Type-1 for Activation in an Epithelial Cell Expression Model
• Activation of a Membrane-Bound Serine Protease Matriptase on the Cell Surface
• Role of the Stem Domain of Matriptase in the Interaction with its Physiological Inhibitor, Hepatocyte Growth Factor Activator Inhibitor Type I
• Reduced Urination Rate while Drinking Beer with an Unpleasant Taste and Off-flavor
• Correlation between the Drinkability of Beer and Gastric Emptying
• Preference for Dried Bonito Broth in Olfactory-Blocked or Taste Nerve-Sectioned Mice in the Two-Bottle Choice Test
• The Occurrence of Matriptase C-Terminal Fragments on the Apical and Basolateral Sides of Madin–Darby Canine Kidney Epithelial Cells
• Effects of CH-19 Sweet, a Non-Pungent Cultivar of Red Pepper, on Sympathetic Nervous Activity, Body Temperature, Heart Rate, and Blood Pressure in Humans
• Effects of CH-19 Sweet, a Non-Pungent Cultivar of Red Pepper, in Decreasing the Body Weight and Suppressing Body Fat Accumulation by Sympathetic Nerve Activation in Humans
• Why Is Fat so Tasty? Chemical Reception of Fatty Acid on the Tongue
• Improved Swimming Pool Achieves Higher Reproducibility and Sensitivity to Effect of Food Components as Ergogenic Aids
• Effect of Timing of Meal Intake after Squat Exercise Training on Bone Formation in the Rat Hindlimb
• Oleuropein, a Phenolic Compound in Extra Virgin Olive Oil, Increases Uncoupling Protein 1 Content in Brown Adipose Tissue and Enhances Noradrenaline and Adrenaline Secretions in Rats
• Effects of Capsaicin Pretreatment on 2-Deoxy-D-glucose- and Acetylcholine-induced Catecholamine Secretion from the Adrenal Medulla(Food & Nutrition)
• Enhancement by Capsaicin of Energy Metabolism in Rats through Secretion of Catecholamine from Adrenal Medulla(Food & Nutrition)
• Ultrasonography Evaluation of Abdominal Fat in Live Rats
• Effect of Meal Timing after Resistance Exercise on Hindlimb Muscle Mass and Fat Accumulation in Trained Rats
• Inhibition by a Capsaicin Antagonist (Capsazepine) of Capsaicin-induced Swimming Capacity Increase in Mice
• Increase in Swimming Endurance Capacity of Mice by Capsaicin-induced Adrenal Catecholamine Secretion
• Allylthiamindisulfide and Related Compounds Enhance Thermogenesis with Increasing Noradrenaline and Adrenaline Secretion in Rats
• Characteristic and Localization of the Monitor Peptide Receptor
• Tissue Distribution of the Enzymes Concerned with the Biosynthesis of NAD in Rats(Biological Chemistry)
• Comparison of the Enzyme Activities in the Tryptophan-NAD Pathway between the Wistar and Sprague Dawley Strains of Rats
• Capsiate, a Nonpungent Capsaicin Analog, Increases Endurance Swimming Capacity of Mice by Stimulation of Vanilloid Receptors
• A Sports Drink Based on Highly Branched Cyclic Dextrin Generates Few Gastrointestinal Disorders in Untrained Men during Bicycle Exercise
• Effects of Physical Fatigue in Mice on Learning Performance in a Water Maze
• Amino Acid Composition of Soybean Protein Increased Postprandial Carbohydrate Oxidation in Diabetic Mice(Food & Nutrition Sience)
• Protocol for Measuring the Endurance Capacity of Mice in an Adjustable-current Swimming Pool(Food & Nutrition Science)
• Use of ^C-labeled Glucose for Measuring Exogenous Glucose Oxidation in Mice(Food & Nutrition Science)
• Administration of Capsiate, a Non-Pungent Capsaicin Analog, Promotes Energy Metabolism and Suppresses Body Fat Accumulation in Mice(Food & Nutrition Science)
• Increased Energy Metabolism and Suppressed Body Fat Accumulation in Mice by a Low Concentration of Conjugated Linoleic Acid(Food & Nutrition Science)
• CH-19 Sweet, a Non-Pungent Cultivar of Red Pepper, Increased Body Temperature and Oxygen Consumption in Humans(Food & Nutrition Science)
• CH-19 Sweet, Nonpungent Cultivar of Red Pepper, Increased Body Temperature in Mice with Vanilloid Receptors Stimulation by Capsiate
• Inhibitory Effect of Monoacylglycerol on Fatty Acid Uptake into Rat Intestinal Epithelial Cells
• Antidiabetic Effect of Glycyrrhizin Genetically Diabetic KK-A^y Mice
• Elevation of Blood NAD Level after Moderate Exercise in Young Women and Mice
• Capsaicin Increases Modulation of Sympathetic Nerve Activity in Rats : Measurement Using Power Spectral Analysis of Heart Rate Fluctuations
• Biochemical and Physiological Characteristics of Fat Cell
• Oleyl Alcohol Inhibits Intestinal Long-Chain Fatty Acid Absorption in Rats
• Enhancement of Swimming Endurance in Mice by Highly Branched Cyclic Dextrin
• Lowering Effect of Phenolic Glycosides on the Rise in Postprandial Glucose in Mice
• The Structural Requirements of Matriptase in Its Ectodomain Release in Polarized Epithelial Cells
• Autonomic Nervous Responses According to Preference for the Odor of Jasmine Tea(Food & Nutrition Science)
• (-)-Hydroxycitrate Ingestion and Endurance Exercise Performance
• Aversive Sensation in the Brain after Eating Unpalatable Food
• Production of a Soluble Recombinant Prion Protein Fused to Blue Fluorescent Protein without Refolding or Detergents in Escherichia coli Cells
• Mechanism for the Increased Defecation and Jejunum Mucosal Protein Content in Rats by Feeding Germinated Barley Foodstuff
• Effects of CH-19 Sweet, a Non-Pungent Cultivar of Red Pepper, on Sympathetic Nervous Activity, Body Temperature, Heart Rate, and Blood Pressure in Humans
• The Structural Requirements of Matriptase in Its Ectodomain Release in Polarized Epithelial Cells
• Matriptase Does Not Require Hepatocyte Growth Factor Activator Inhibitor Type-1 for Activation in an Epithelial Cell Expression Model
• The Occurrence of Matriptase C-Terminal Fragments on the Apical and Basolateral Sides of Madin-Darby Canine Kidney Epithelial Cells
• The Activity of a Type II Transmembrane Serine Protease, Matriptase, Is Dependent Solely on the Catalytic Domain
• Granzyme A Causes Detachment of Alveolar Epithelial A549 Cells Accompanied by Promotion of Interleukin-8 Release
• A Lymphocyte Serine Protease Granzyme A Causes Detachment of a Small-Intestinal Epithelial Cell Line (IEC-6)
• A Role of a Lymphocyte Tryptase, Granzyme A, in Experimental Ulcerative Colitis
• Sulfated Polysaccharides Derived from Dietary Seaweeds Increase the Esterase Activity of a Lymphocyte Tryptase, Granzyme A
• Inhibition of Membrane-Type Serine Protease 1/Matriptase by Natural and Synthetic Protease Inhibitors
• Non-Pungent Capsaicin Analogs (Capsinoids) Increase Metabolic Rate and Enhance Thermogenesis via Gastrointestinal TRPV1 in Mice
• Capsinoids, Non-Pungent Capsaicin Analogs, Reduce Body Fat Accumulation without Weight Rebound unlike Dietary Restriction in Mice
• Intragastric Administration of TRPV1, TRPV3, TRPM8, and TRPA1 Agonists Modulates Autonomic Thermoregulation in Different Manners in Mice
• The optimal activity of a pseudozymogen form of recombinant matriptase under the mildly acidic pH and low ionic strength conditions.
• A recombinant catalytic domain of matriptase induces detachment and apoptosis of small-intestinal epithelial IEC-6 cells cultured on laminin-coated surface
• Effect of Neural Blockers and CR-1392 on the Pancreatic Enzyme Secretion in Response to Soybean Trypsin Inhibitor and Hydrochloric Acid(Food & Nutrition)
• Selective Assay Method with Use of Heat Stability for a Trypsin-sensitive Cholecystokinin (CCK)-releasing Peptide (Monitor Peptide) in Rat Bile-pancreatic Juice(Biological Chemistry)
• Requirement for Pancreatic Juice in the Feedback Regulation of Pancreatic Enzyme Secretion in Swine: Evidence for the Occurrence of a Regulatory Factor in the Pancreatic Juice(Food & Nutrition)
• Preference for Dried Bonito Broth in Olfactory-Blocked or Taste Nerve-Sectioned Mice in the Two-Bottle Choice Test
• Recognition System for Dietary Fatty Acids in the Rat Small Intestine
• The Recognition System of Dietary Fatty Acids by the Rat Small Intestinal Cells
• Non-uniform Decrease of Nicotinamide in Various Tissues of Rats Fed on a Niacin-free and Tryptophan-limited Diet(Food & Nutrition)
• Roles as metabolic regulators of the non-nutrients, capsaicin and capsiate, supplemented to diets
• Computer assisted design for the implantable left ventricular assist device (LVAD) blood pump using computational fluid dynamics (CFD) and computer-aided design and manufacturing (CAD/CAM)
• Relationship between the Preference for Sake (Japanese rice wine) and the Movements of Metabolic Parameters Coinciding with Sake Intake
• Effects of Fasting on the Blood NAD Level, and Urinary Excretion of 5-Hydroxyindole-3-acetic Acid, and Nicrotinamide and Its Metabolites in Rats (Food & Nutrition)
• Effect of Dietary Protein Levels on the Urinary Excretion of Nicotinamide and Its Metabolites in Rats(Food & Nutrition)
• Inhibition of Membrane-Type Serine Protease 1/Matriptase by Natural and Synthetic Protease Inhibitors
• Intake of Dried Bonito Broth Flavored with Dextrin Solution Induced Conditioned Place Preference in Mice
• Increased Noradrenergic Activity in the Ventromedial Hypothalamus during Treadmill Running in Rats
• Blood Lactate Functions as a Signal for Enhancing Fatty Acid Metabolism during Exercise via TGF-β in the Brain
• Intragastric Administration of Allyl Isothiocyanate Reduces Hyperglycemia in Intraperitoneal Glucose Tolerance Test (IPGTT) by Enhancing Blood Glucose Consumption in Mice
• Effects of Aroma Components from Oxidized Olive Oil on Preference
• Increased Levels of Extracellular Dopamine in the Nucleus Accumbens and Amygdala of Rats by Ingesting a Low Concentration of a Long-Chain Fatty Acid
• Digestion of α-Lactalbumin in Rat Gastrointestinal Tracts
• Investigation of Digestion and Absorption of a Dietary Protein by Using Its Antigenicity as an Index: Gastrointestinal Digestion of Ovalbuminf
• Effects of Dietary Casein and Adrenal Hormone on the Dietary Induction of α-Amino-β-carboxymuconate-ε-semialdehyde Decarboxylase Activity in Rat
• Stimulation of Rat Pancreatic EnzymeSecretion by Diet Components
• A Synthetic Peptide-Based Assay System for Detecting Binding between CD36 and an Oxidized Low-Density Lipoprotein
• Assessment of Key Amino-Acid Residues of CD36 in Specific Binding Interaction with an Oxidized Low-Density Lipoprotein

もっと見る 閉じる

スポンサーリンク