A Lymphocyte Serine Protease Granzyme A Causes Detachment of a Small-Intestinal Epithelial Cell Line (IEC-6)

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概要

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• Granzyme A (GzmA) is a serine protease (trypsin-like specificity) produced in cytotoxic lymphocytes. This enzyme is believed to enter virus-infected cells and growing tumors and induce apoptosis, but the roles of GzmA expressed in lymphocytes scattered through the epithelial layer of the normal small intestine are unknown. In the present study, recombinant rat GzmA (rGzmA) was found to cause morphological changes and detachment of a non-transformed rat small-intestinal epithelial cell line IEC-6, although the rGzmA-treated cells detached as aggregates with no changes characteristic of apoptosis. rGzmA-induced deformation and detachment occurred in IEC-6 cells plated with collagen type IV and fibronectin, but not in those plated with laminin. These findings suggest that GzmA in the normal small intestine participates in the reduction of adhesion between epithelial cells and basement membranes, through its ability to cleave extracellular matrix components.

• 社団法人 日本農芸化学会の論文
• 2008-09-23

著者

• Fushiki Tohru

  Laboratory Of Nutrition Chemistry Division Of Food Science And Biotechnology Graduate School Of Agri

• Fushiki Tohru

  Kyoto Univ. Kyoto City Jpn

• Tsuzuki Satoshi

  Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Ag

• Fushiki T

  Laboratory Of Nutrition Chemistry Division Of Food Science And Biotechnology Graduate School Of Agri

• Tsuzuki Satoshi

  Laboratory Of Nutrition Chemistry Division Of Food Science And Biotechnology Graduate School Of Agri

• YOSHIKAWA Yumiko

  Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Ag

• HIRAYASU Hirofumi

  Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Ag

• Fushiki Tohru

  Laboratory Of Nutrition Chemistry Division Of Food Science And Biotechnology Graduate School Of Agri

• Fushiki Tohru

  Laboratory Of Nutrition Chemistry Division Of Food Science And Biotechnology Graduate School Of Agri

• Hirayasu Hirofumi

  Laboratory Of Nutrition Chemistry Division Of Food Science And Biotechnology Graduate School Of Agri

• Fushiki Tohru

  Laboratory Of Nutrition Chemistry Division Of Applied Life Sciences Graduate School Of Agriculture K

• Yoshikawa Yumiko

  Laboratory Of Nutrition Chemistry Division Of Food Science And Biotechnology Graduate School Of Agri

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