Purification and Characterization of a Morphine UDP-Glucuronyltransferase Isoform from Untreated Rat Liver
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概要
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A morphine UDP-glucuronyltransferase was purified from liver microsomes of untreated Sprague-Dawley rats. A new gel, ω-(β-carboxypropionylamino)octyl Sepharose 4B, was prepared by coupling monomethylsuccinate with ω-aminooctyl Sepharose 4B and this was used as an efficient tool for the separation of microsomal enzymes. Emulgen 911 solubilized microsomes were applied to a column packed with the gel and eluted at pH 7.4 while increasing KCl concentration in a stepwise manner. An isoform was further purified with UDP-hexanolamine Sepharose 4B gel. The purified UDP-glucuronyltransferase (morphine UGT of untreated rat, morphine UGTUT) exhibited a molecular weight of 52000 on sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and was capable of glucuronidating the 3-hydroxyl group of morphine. The isoform catalyzed to a small extent the glucuronidation of 4-hydroxybiphenyl; however, no glucuronidation activity towards androsterone, testosterone, bilirubin, 4-nitrophenol and the 6-hydroxyl group of morphine was observed. The difference in properties, compared with morphine UGT (molecular weight 56000)which was purified previously from phenobarbital-treated rats, is discussed.
- 社団法人日本薬学会の論文
- 1993-08-15
著者
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小栗 一太
九州大学 院 薬
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小栗 一太
九大院・薬
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小栗 一太
九州大学薬学部衛生化学裁判化学教室
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吉村 英敏
Department of Food and Nutrition, Nakamura Gakuen University
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小栗 一太
Faculty of Pharmaceutical Sciences, Kyushu University
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吉村 英敏
中村学園大・食物栄養
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石井 祐次
Faculty of Pharmaceutical Sciences, Kyushu University 62
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石井 祐次
茨城大・社会医・環境医
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Yoshimura Hidetoshi
Department Of Food And Nutrition Nakamura Gakuen University
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吉村 英敏
Department Of Food And Nutrition Nakamura Gakuen University
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