Detection of Morphine in Urine. II. An Improved Method by Thin-Layer Chromatography Utilizing Potassium Platinum Iodide as the Reagent for Both Coloration and Fluorescence

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A simple and reliable identification method of morphine was developed by successive performance of color and fluorescent reactions with potassium platinum iodide reagent utilizing the same thin-layer chromatogram; after separation by thin-layer chromatography morphine could be identified as a violet-colored spot with above reagent and then reconfirmed as a blue fluorescent spot under ultraviolet light after the chromatogram was exposed to ammonia vapor. The fluorometric identification is based on the essentially same principal to that of Kupferberg, et al.^<1,2)>, however the present method has certain advatages to identify morphine, especially excreted in urine, in respect of simplicity and reliability.
公益社団法人日本薬学会の論文
1966-11-25