工業微生物の対酸素挙動の解析 : 1. 酵母の酸素適応速度および代謝におよぼす酸素濃度の影響

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1) A convenient method is described for analyzing attenuation curve of dissolved oxygen obtained polarographically with cell suspension.2) The fitness of the respiration rate-[O_2] relationship for the MICHAELIS function has been found to be fairly good with areobically grown cells of various species. The K_m values are mostly of the magnitude of 10^<-6>M/l. But with anarobically grown cells of Saccharomyces cerevisiae, an irregular pattern of complex nature was observed. Fluctuation of K_m values is discussed.3) Oxygen concentration applied to a culture seems to affect the K_m value of cells grown therein : with lower oxygen concentration, the cells having lower value of K_m are obtained.4) The specific reates of oxgen adapiation have been surveyed at various levels of oxygen concentration starting with anaerobically grown of Sacch. cerevisiae. Evidance has been presented that the above adaptation rate-[O_2] relationship obeys well the MICHAELIS rule, as tested by LINEWEAVER-BURK. The K_s value, the saturation constant for adaptation, obtained from the plots about tenfold larger than the K_m value for respiration. Reasonable expression of the specific rate of adaptation is discussed.5) It has been suggested also that the final level of respiratory activity attained is a function of oxygen concentratio, which resembles the MICHAELIS function.6) The specific growth rate (μ) of cells of Sacch. cerevisiae already adapted to oxygen to a considerable extent has an optimum in regard to oxygen concentration at 1.16×10^<-4> m/l. Such a concentration optimum is not observed in the case of Candida utilis. The cause of the observed optimum is discussed.
社団法人日本生物工学会の論文
1960-06-15

工業微生物の対酸素挙動の解析 : 1. 酵母の酸素適応速度および代謝におよぼす酸素濃度の影響

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