Existence of an Optically Heterogeneous Peptide Unit in Poly(γ-Glutamic Acid) Produced by Bacillus subtilis
スポンサーリンク
概要
- 論文の詳細を見る
Poly(γ-glutamic acid) (PGA) produced by Bacillus subtilis F2-01 on an industrial scale was ultimately depolymerized into a peptide fraction (F-I) and a γ-glutamyl oligopeptide fraction (F-II) by the action of fungal PGA-hydrolase (γ-L-glutamyl hydrolase). F-I consisted of both D- and L-glutamic acid (76 : 24), whereas L-glutamic acid alone was detected with F-II. Incomplete depolymerization of PGA depended on the presence of D- and L-glutamic acid residues in F-I but not on end product inhibition or denaturation of the enzymw. Analyses by ultracentrifugation supported the loss of intermolecular interaction between γ-glutamyl peptides that may interfere with the enzymatic hydrolysis of F-I by accelerating aggregate formation. F-I is proposed to exist as an optically heterogeneous peptide unit in PGA, in which D- and L-glutamic acid isomers are copolymerized into a single chain.
- 公益社団法人日本生物工学会の論文
- 1997-10-25
著者
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Tano Tatsuo
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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田中 孝明
新潟大・工・機能材料
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Taniguchi M
Graduate School Of Science Osaka City University
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Fujita K
Tohoku Univ. Sendai Jpn
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Taniguchi Makoto
Graduate School Of Science Osaka City University
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Fujita Ken-ichi
Department Of Biology And Geosciences Graduate School Of Science Osaka City University
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Takenishi Shigeyuki
Osaka Municipal Technical Research lnstitute
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Tanaka Toshio
Department Of Cardiology Matsusaka Central General Hospital
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Taniguchi M
Taiyo Kagaku Co. Ltd. Mie Jpn
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Taniguchi Makoto
Department Of Bio- And Geoscience Gradaute School Of Science Osaka City University
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Tanaka Toshio
Department Of Applied Physics Faculty Of Engineering Tokyo University
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Takenishi Shigeyuki
Osaka Municipal Technical Research Institute
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Tanaka Toshio
Department of Applied Chemistry, Faculty of Engineering, Osaka Univeristy
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TANIGUCHI Makoto
Department Medical Research Institute Kanazawa Medical University
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