The Use of Mammalian Cultured Cells Loaded with a Fluorescent Dye Shows Specific Membrane Penetration of Undissociated Acetic Acid
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概要
- 論文の詳細を見る
Acetic acid induces unique physiological responses in mammalian cells. Our previous study found that fura-2-loaded human embryonic kidney (HEK) 293T cells showed a robust intracellular fluorescence response immediately after stimulation with acetic acid, and no such response in the case of citric acid. In the present study, we aimed to identify the unique characteristics of acetic acid responsible for this phenomenon. We found that one such feature is its hydrophobicity. We also discovered that acetic acid induces cell responses by intracellular acidification. Of the components of acetic acid in solution (protons, acetate ions, and undissociated acetic acid), undissociated acetic acid might be the functional unit that penetrates the lipid bilayer of cell membranes to acidify the intracellular environment, thereby inducing cell responses. The method used in this study might be convenient in evaluating the intracellular acidification of cultured cells by acids in the external environment.
- Japan Society for Bioscience, Biotechnology, and Agrochemistryの論文
- 2012-03-23
著者
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Kishi Mikiya
Central Res. Inst. Mizkan Group Corp.
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Misaka Takumi
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Okada Shinji
Graduate School Of Agricultural And Life Sciences Department Of Applied Biological Chemistry The Uni
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Okada Shinji
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Misaka Takumi
Dep. Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Univ. Of
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Okada Shinji
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Misaka Takumi
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Abe Keiko
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Abe Keiko
Department Of Agricultural Chemistry The University Of Tokyo
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Yamagami Keigo
Central Res. Inst. Mizkan Group Co.
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ISHII Sho
Central Research Institute, Mizkan Group Co.
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Ishii Sho
Central Research Institute Mizkan Group Co.
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Yamagami Keigo
Central Research Institute Mizkan Group Co.
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Kishi Mikiya
Central Research Institute Mizkan Group Co.
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Abe Keiko
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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