鶏卵中の抗生物質検出のための従来法の適用について

概要

論文の詳細を見る
To establish a routine method for the detection of antibiotics in egg, both paper disk assay method and TTC test were applied. In case of TTC test, to suppress the antimicrobial action of egg white, diluted egg solutions were previously heated after the digestion with a proteolytic enzyme. In case of paper disk assay method, both whole egg and egg white were used directly, and yolk was used after being slightly diluted with water. Although egg white gave a little high value owing to its antimicrobial action, no adverse effect against paper disk assay method could be found so far as whole egg or diluted yolk was concerned. From the result of this study, paper disk assay method was recommended for the detection of antibiotics in egg. The results of disk assay carried out on seven kinds of antibiotics dissolved in whole egg, yolk or egg white revealed that penicillin and streptomycin were detected at the level of 0.003-0.005 u/ml and 1_??_2 μg/ml, respectively.
社団法人日本農芸化学会の論文