Effects of deoxyribonuclease I and neuraminidase treatments on the specific binding of 3H-prazosin and 3H-quinuclidinyl benzilate (3H-QNB) to .ALPHA.-adrenergic and muscarinic receptors in rat myocardial membranes.
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概要
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The effects of neuraminidase and deoxyribonuclease I (DNase) treatments on the specific binding of <SUP>3</SUP>H-prazosin and <SUP>3</SUP>H-quinuclidinyl benzilate (<SUP>3</SUP>H-QNB) to α-adrenoceptors and muscarinic cholinergic receptors in the membrane of the rat myocardium was examined, and the dissociation constant (K<SUB>d</SUB>) and the maximum number of binding sites (B<SUB>max</SUB>) was analyzed using the method of Scatchard analysis. Although no changes in K<SUB>d</SUB> values were observed when DNA or sialic acid was removed from cardiac muscles by treatments with DNase or neuraminidase, the B<SUB>max</SUB> values of α-adrenergic and muscarinic receptors were markedly decreased after treatment with DNase, while neuraminidase treatment induced an increase in the B<SUB>max</SUB> values of the α-adrenoceptors. The possibility that these results provide the basis for elucidation of the characteristics of these receptors in the rat myocardium was discussed.
- 公益社団法人 日本薬理学会の論文
著者
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Imai Shoichi
Department Of Phamacology Niigata University School Of Medicine
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Nagatomo Takafumi
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Niigata University Of Pharmacy And App
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Tsuchihashi Hiroshi
Department Of Pharmacology Niigata College Of Pharmacy
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