cAMP-Independent Chloride Secretion Activated by a Vasoactive Intestinal Peptide in a Monolayer Culture of Human Bronchinal Epithelial Cells
スポンサーリンク
概要
- 論文の詳細を見る
To investigate the effects of vasoactive intestinal peptide (VIP) on Cl^- transport across normal human bronchial epithelial (NHBE) cells grown in a monolayer, changes in short-circuit current (Isc) were measured in Ussing chamber systems. In the presence of 10^<-4>M amiloride, the addition of VIP to the serosal solution led to an increase in the Isc in a concentration-dependent manner, the 50% effective concentration (EC_<50>) being 2.6×10^<-11>M. However, the addition of 10^<-5>M forskolin had little effect on the increase in Isc. On the other hand, in the intracellular cAMP measurement, 10^<-5>M forskolin remarkably increased the cAMP levels, but 10^<-7>M VIP did not.This result suggests that Cl^- secretion by VIP is not related to the raised intracellular cAMP levels in NHBE cells.
- 公益社団法人日本薬学会の論文
- 1999-07-15
著者
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Nagatomo T
Dep. Of Pharmacology Fac. Of Pharmaceutical Sciences Niigata Univ. Of Pharmacy And Applied Life Scie
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Tokunaga Tatsuhiro
Molecular Chemistry Resarch Laboratories Institute For Drug Discovery Research Yamanouchi Pharmaceut
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KISO Tatsuya
Research Department, Sawai Pharmaceutical Co., Ltd.,
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NAMIKAWA Tomoko
Research Department, Sawai Pharmaceutical Co., Ltd.,
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TOKUNAGA Tetsuhiro
Research Department, Sawai Pharmaceutical Co., Ltd.,
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OHTSUBO Yoshikazu
Research Department, Sawai Pharmaceutical Co., Ltd.,
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Kiso Tatsuya
Research Department Sawai Pharmaceutical Co. Ltd.
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Kakita T
Research Department Sawai Pharmaceutical Co. Ltd.
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Nagatomo Takafumi
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Niigata University Of Pharmacy And App
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Tokunaga T
Institute For Drug Discovery Research Yamanouchi Pharmaceutical Co. Ltd.
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Ohtsubo Yoshikazu
Biological Res. Dep. Sawai Pharmaceutical Co. Ltd.
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Namikawa Tomoko
Biological Res. Dep. Sawai Pharmaceutical Co. Ltd.
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OHTSUBO Yoshikazu
Research Department, Sawai Pharmaceutical Co. Ltd.
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