Binding characteristics of 3H-dihydroalprenolol to .BETA.-adrenergic receptors of rat brain: Influence of lectins.
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概要
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The significance of the carbohydrate moieties of the β-adrenergic receptor molecule in the rat brain was examined using the radioligand binding assay method. Thus, this experiment was designed to assess the effects of lectins, concanavlin A (Con A), <I>Phaseolus vulgaris</I> agglutinin (PHA), and wheat germ agglutinin (WGA) on the affinity of the β-adrenoceptor. The rat brain was used and the β-adrenoceptor binding assay was carried out using <SUP>3</SUP>H-dihydroalprenolol as a ligand. Con A and PHA significantly caused an increase in the values of the density of β-adrenoceptor (B<SUB>max</SUB>) and a reduction in the values of the dissociation constant (K<SUB>d</SUB>), but significant changes were not observed with WGA. These results strongly suggest that the carbohydrate moieties of the cell surface containing the β-adrenoceptor molecule may have a crucial role in the drug-receptor interaction, and they imply that the β-adrenoceptor molecule is a glycoprotein which contains N-linked carbohydrate chains.
- 公益社団法人 日本薬理学会の論文
著者
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Nagatomo Takafumi
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Niigata University Of Pharmacy And App
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Tsuchihashi Hiroshi
Department Of Pharmacology Niigata College Of Pharmacy
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