Brevibacterium linensの生産する菌体外プロテアーゼについて-1-プロテアーゼの生産性および諸性質〔英文〕

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In the present study the production of extracellular protease by Brevibacterium linens and some properties of the extracellular protease produced were investigated.Of the media experimented, medium-D composed of yeast extract 1%, peptone 1%, NaCl 1%, K2HPO4 1% and casein 2% was found to be the most preferable for the production of the extracellular protease. The extracellular protease was prepared from medium-D after 24 hours' growth of Brev. linens at 20°C by ammonium sulfate precipitation. Protease was purified ca. 18 times above that of the original enzyme (culture filtrate) by Sephadex G-100 column chromatography, and enzymatic properties of it were studied.The optimum pH of the protease was 7.0 in phosphate buffer. The optimum temperature was 25°C, and the inactivation of the extracellular protease was observed to begin already even at 40°C, and ten minutes' heating at 50°C resulted in camplete loss of activity. The extracellular protease hydrolyzed actively milk casein and bovine haemoglobin, while unheated egg albumin was slightly hydrolyzed, and egg albumin heated at 95°C for 10 minutes was hardly hydrolyzed by this protease.
社団法人日本畜産学会の論文