うっ滞性乳腺炎動物モデルの作成

概要

論文の詳細を見る
Mastitis one of the disease attack mothers during the lactation period. Especially, it is assumed that stagnation mastitis is caused by lacteal discharge disorder, and conceivably, meals are the principal risk factor. Establishment of animal experiment method was tried to inspect efficiently meal components in association with the mastitis onset. At first, the mammary gland tissues were stained with hematoxylin and eosin after the pups were separated from the mother mouse during a lactation period for forced weaning. Inflammation such as epithelial cells shedding, humoral shift to interstitium and invasion of mononuclear cells to interstitium were confirmed after 96 hours from weaning. In addition, measuring the mast cell count in order to evaluate inflammatory degree indicated that the cell count increased significantly in mammary gland tissue of forced weaning mice in comparison with continued nursing mice. The stagnation mastitis induction model made by extremely easy operation of forced weaning will give an experimental tool to elucidate relation between mastitis and diets.乳腺炎は授乳期の母親の疾病の一つで, なかでもうっ滞性乳腺炎は乳汁の排出障害が原因とされており, その発症危険因子として食餌が疑われている. 効率よく食餌成分と乳腺炎発症との関連を検証するために動物実験系の構築を試みた. 先ず, 授乳期の親マウスから仔マウスを強制離乳させ乳腺組織を観察した結果, 離乳96時間後に上皮細胞の脱落や間質への体液の移行, 問質への単核細胞の浸潤などの炎症が確認された. また, 炎症の度合いを数値化するためにマスト細胞数を計測した結果, 離乳マウスの乳腺組織では有意に細胞数が増加していた. 極めて容易な強制離乳により作成できたうっ滞性乳腺炎誘導モデルは, 食餌成分と乳腺炎との関連解明について実験的な研究手法を与えるものである.
宮崎大学の論文
2009-01-30

うっ滞性乳腺炎動物モデルの作成

スポンサーリンク

概要

著者

関連論文

スポンサーリンク