Characterization of an immortalized hepatic stellate cell line established from metallothionein-null mice
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概要
- 論文の詳細を見る
Hepatic stellate (HS) cells were isolated from the livers of metallothionein (MT)-null and control mice and used to establish IMS/MT(-) and IMS/N cell lines, respectively, using SV40 virus transformation. Cellular morphology, incorporation of vitamin A and expression α-SMA, desmin and SV40 T-antigen were used to confirm that both cell lines were immortal HS cells. The growth rates of both cell lines were similar and there was little difference between cell line sensitivity to zinc. MT-null IMS/MT(-) cells were more sensitive to cadmium and mercury, although both cell lines accumulated almost equal amounts of cadmium during a 24-hr culture period. As HS cells play an important role in hepatic fibrosis and are activated by heavy metals such as cadmium or reactive oxygen, the MT-null HS cell line derived in this study should be a useful experimental model for examination of the role of MT in HS cell activation.
- 日本トキシコロジー学会の論文
- 2006-10-23
著者
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Naganuma Akira
Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Toho
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HASEGAWA Tatsuya
Department of Environmental Biochemistry, Yamanashi Institute of Environmental Sciences
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SEKO Yoshiyuki
Department of Environmental Biochemistry, Yamanashi Institute of Environmental Sciences
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Seko Yoshiyuki
Department Of Environmental Biochemistry Yamanashi Institute Of Environmental Sciences
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Kaji Toshiyuki
Department Of Environmental Science Faculty Of Pharmaceutical Sciences Hokuriku University
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Naganuma Akira
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Sciences Tohoku
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Kaji Toshiyuki
Department Of Environmental Health Faculty Of Pharmaceutical Sciences Hokuriku University
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MIURA Nobuhiko
Division of Health Effects Research, National Institute of Industrial Health Nagao
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KANAYAMA Yoshitaka
Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Toho
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NAGAI Wakako
Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Toho
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Hasegawa Tatsuya
Department Of Environmental Biochemistry Yamanashi Institute Of Environmental Sciences
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Nagai Wakako
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Sciences Tohoku
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Naganuma Akira
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Science Tohoku
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Kanayama Yoshitaka
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Sciences Tohoku
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Naganuma Akira
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Sciences Tohoku
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Miura Nobuhiko
Division Of Health Effects Research Japan National Institute Of Occupational Safety And Health
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Miura Nobuhiko
Div. Of Health Effects Res. Japan National Inst. Of Occupational Safety And Health
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