Proteoglycans Predominantly Synthesized by Human Brain Microvascular Endothelial Cells in Culture are Perlecan and Biglycan
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概要
- 論文の詳細を見る
It has been suggested that the properties of vascular endothelial cells differ depending on the type of blood vessel. Proteoglycans are macromolecules that contribute to vascular properties through regulation of endothelial cell functions. In this study, we characterized proteoglycans synthesized by cultured human brain microvascular endothelial cells by biochemical techniques. The experiments indicated that the cells synthesize and secrete two types of proteoglycans : large heparan sulfate proteoglycans bearing heparan sulfate chains of approximately M_r〜68000 and chondroitin/dermatan sulfate proteoglycans bearing chondroitin/dermatan sulfate chains of approximately M_r〜48000. The heparan and chondroitin/dermatan sulfate proteoglycans were identified as perlecan and biglycan, respectively, by Western blot analysis. A part of perlecan was associated with the cell layer, while most of biglycan was secreted into the medium. These results suggest that the characteristics of proteoglycans synthesized by brain microvascular endothelial cells are similar to the characteristics of those synthesized by previously reported arterial endothelial cells. Regulation of endothelial cell functions by proteoglycans may be independent of blood vessel types.
- 社団法人日本薬学会の論文
- 2005-10-01
著者
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DENG Xingyun
Department of Human Movement and Health, Graduate School of Medical Science, Kanazawa University
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FUJIWARA Yasuyuki
Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University
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Fujiwara Yasuyuki
北陸大学 薬学部環境健康学
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Fujiwara Yasuyuki
Lab. Of Pharmaceutical Health Sciences School Of Pharmacy Aichi Gakuin Univ.
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Kaji T
Organization For Frontier Research In Preventive Pharmaceutical Sciences Hokuriku University
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Kaji Toshiyuki
Organization For Frontier Res. In Preventive Pharmaceutical Sciences Hokuriku Univ.
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Kaji Toshiyuki
Department Of Environmental Science Faculty Of Pharmaceutical Sciences Hokuriku University
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Yamamoto Chika
Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University
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Fujiwara Yasuyuki
Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University
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Kaji Toshiyuki
Department Of Environmental Sciences Faculty Of Pharmaceutical Sciences Hokuriku University
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Kaji Toshiyuki
Department Of Environmental Health Faculty Of Pharmaceutical Sciences:organization For Frontier Rese
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Kaji Toshiyuki
Department Of Environmental Health Faculty Of Pharmaceutical Sciences Hokuriku University
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Yamamoto C
Organization For Frontier Research In Preventive Pharmaceutical Sciences Hokuriku University
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Yamamoto Chika
Department Of Environmental Health Faculty Of Pharmaceutical Sciences Hokuriku University
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Fujiwara Yasuyuki
Dep. Of Environmental Health Fac. Of Pharmaceutical Sciences Hokuriku Univ.
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Fujiwara Yasuyuki
Department Of Environmental Health Faculty Of Pharmaceutical Sciences
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Fujiwara Yasuyuki
Department Of Cardiovascular Surgery Seijunkai Johoku Hospital
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Kaji T
北陸大学薬学部:北陸大学学術フロンティア研究組織
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Deng Xingyun
Department Of Environmental Health Faculty Of Pharmaceutical Sciences
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Yamamoto Chika
Organization Of Frontier Research For Preventive Pharmaceutical Science Hokuriku University:departme
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