Optimum Conditions for Derivatization of Glutathione, Cysteine and Cysteinylglycine in Human Plasma with Ammonium 7-Fluorobenzo-2-Oxa-1,3-Diazole-4-Sulfonate for Accurate Quantitation by High-Performance Liquid Chromatography
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概要
- 論文の詳細を見る
For the accurate quantitation of glutathione (GSH), cysteine (Cys) and cysteinylglycine (CysGly) in human plasma by detection of fluorescence after derivatization with ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD-F), a thiol-specific fluorogenic agent and high-performance liquid chromatography (HPLC), we attempted to identify optimum conditions for the derivatization and treatment of samples. The extent of derivatization with SBD-F of endogenous thiol compounds in human plasma was affected by several factors, such as the concentration of SBD-F and the reaction time. Acidification and deproteinization of plasma immediately after the separation of plasma from whole blood were essential and prevented the determination of inappropriately low values. The Inethod developed in the present study allowed recovery that was closed to 100% for each compound. The mean values determined for 61 healthy individuals were as follows: Cys, 50.1μM; CysGly, 12.2μM; and GSH, 5.3μM.
- 公益社団法人日本薬学会の論文
- 2003-12-01
著者
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TAKAHASHI Katsuyuki
Department of Obstetrics and Gynecology, Sendai National Hospital
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Izumi Toru
Department Of Cardio-angiology Kitasato University School Of Medicine
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Makimura Mizue
Department Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences University Of Tokyo:(present
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Makimura Mizue
Department Of Pharmacy University Of Nihon Itabashi Hospital
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Naganuma Akira
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Science Tohoku
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Izumi T
Department Of Emergency And Critical Care Medicine University Of Nihon Itabashi Hospital
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Miyairi Shinichi
Laboratory Of Bio-organic Chemistry College Of Pharmacy Nihon University
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Miyairi Shinichi
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Sciences Tohoku
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Yoshida Shozo
Department Of Emergency And Critical Care Medicine University Of Nihon Itabashi Hospital
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Yoshida Yoshikazu
Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Toho
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Ohiwa Yasuharu
Department of Pharmacy, University of Nihon Itabashi Hospital
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Shimamura Masao
Common-Use Facilities for Medical Research, Nihon University School of Medicine
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Naganuma Akira
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Sciences Tohoku
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Takahashi Katsuyuki
Department Of Clinical Chemistry University Of Nihon Itabashi Hospital
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Ohiwa Yasuharu
Department Of Pharmacy University Of Nihon Itabashi Hospital
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Shimamura Masao
Common-use Facilities For Medical Research Nihon University School Of Medicine
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Yoshida Yoshikazu
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Sciences Tohoku
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Takahashi Katsuyuki
Department of Cardiology, Kurashiki Central Hospital
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TAKAHASHI Katsuyuki
Department of Applied Chemistry, Waseda University
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