siRNA-mediated AMPKα1 subunit gene PRKAA1 silencing enhances methylmercury toxicity in HEK293 cells
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概要
- 論文の詳細を見る
The environmental pollutant methylmercury is a potent neurotoxin. The mechanisms of toxicity and biological defense remain largely unknown. We found that inhibiting the expression of PRKAA1 (AMPKα1), an activated subunit of AMP-activated protein kinase (AMPK), increased susceptibility of HEK293 cells to methylmercury toxicity. Treatment of the cells with AICAR (5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside), an AMPK activator, reduced the methylmercury toxicity. Here, we suggest for the first time that the activation (phosphorylation) of AMPK may play an important role in reducing the toxicity of methylmercury.
- 2010-08-01
著者
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Takahashi Tsutomu
Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Toho
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Naganuma Akira
Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Toho
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Kuge Shusuke
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Sciences Tohoku
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Kuge Shusuke
東北大学 薬研究 生体防御薬
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Tobita Mayumi
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Sciences Tohoku
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HWANG Gi-Wook
Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Toho
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KITA Kayoko
Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Toho
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Hwang Gi-wook
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Sciences Tohoku
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Naganuma A
Tohoku Univ. Sendai Jpn
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Kita Kayoko
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Sciences Tohoku
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Naganuma Akira
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Science Tohoku
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Takahashi Tsutomu
Laboratory Of Kampo-pharmaceutics Faculty Of Pharmacy Osaka Ohtani University
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Naganuma Akira
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Sciences Tohoku
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Naganuma Akira
Department Of Public Health And Molecular Toxicology School Of Pharmaceutical Sciences Kitasato Univ
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Hwang Gi-wook
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Sciences Tohoku
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Takahashi Tsutomu
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Sciences Tohoku
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Takahashi Tsutomu
Laboratory Of Molecular And Biochemical Toxicology Graduate School Of Pharmaceutical Sciences Tohoku
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