A SIMPLE AND RAPID METHOD FOR PREPARATION OF ^<203>HgLABELED METHYLMERCURY FROM ^<203>HgCl_2 AND METHYLCOBALAMIN
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概要
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A rapid and simple method for preparation of ^<203>Hg-labeled methylmercuric chloride (CH_3^<203>HgCl) from ^<203>HgCl_2 and methylcobalamin is described. More than 99% of ^<203>Hg was methylated with methylcobalaminin 0.01N HCl during 1h. CH_3^<203>HgCl formed during the reaction was repidly and completely separated from cobalamins and unreacted ^<203>HgCl_2 by CM-Sephadex C-25 minicolumn. The low cost procedure for preparation of CH_3^<203>HgCl can be completed within 2h and yields an inorganic ^<203>Hg-free CH_3^<203>HgCl which is useful in methylmercury toxicology.
- 公益社団法人日本薬学会の論文
著者
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Imura Nobumasa
Department of Public Health and Molecular Toxicology, School of Pharmaceutical Sciences, Kitasato Un
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Imura Nobumasa
Dep. Of Public Health And Molecular Toxicology School Of Pharmaceutical Sciences Kitasato Univ.
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Imura Nobumasa
Department Of Public Health And Molecular Toxicology School Of Pharmaceutical Sciences Kitasato Univ
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NAGANUMA Akira
Department of Public Health and Molecular Toxicology, School of Pharmaceutical Sciences, Kitasato Un
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Naganuma Akira
Department Of Molecular And Biochemical Toxicology Faculty Of Pharmaceutical Sciences Tohoku Univers
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Urano Tsutomu
Department Of Public Health School Of Pharmaceutical Sciences Kitasato University
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Urano T
Department Of Analytical Chemistry School Of Pharmaceutical Sciences Kitasato University
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Imura N
Department Of Public Health And Molecular Toxicology School Of Pharmaceutical Sciences Kitasato Univ
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Naganuma A
Tohoku Univ. Sendai Jpn
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Urano Tsutomu
Department Of Analytical Chemistry School Of Pharmaceutical Sciences Kitasato University
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Imura Nobumasa
Department Of Public Health And Molecular Toxicology School Of Pharmaceutical Sciences Kitasato Univ
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