METHYLMERECURY-INDUCED MICROTUBULE DEPOLYMERIZATION LEADS TO INHIBITION OF TUBULIN SYNTHESIS
スポンサーリンク
概要
- 論文の詳細を見る
Methylmercury (MeHg) specifically depolymerizes microtubules and inhibits cell proliferation in mouse glioma cells. The effect of microtubule depolymerization by MeHg on tubulin synthesis was studied. Tubulin synthesis analyzed by two-dimensional electrophoresis using [^<35>S] methionine as a tracer was markedly inhibited in mouse glioma cells exposed to 5×10^<-6>M MeHg for 3 hr, which completely depolymerized microtubules. Under this condition, density of the protein bands other than tubulin in autoradiogram remained unchanged on gradient urea-polyacrylamide gels. Furthermore, the decrease in tubulin mRNA level was relevant to that in tubulin synthesis, although action mRNA levels remaind unchanged. In addition, specific transcription rates of β-tubulin genes appeared to be unaffected under the same experimental condition as above. Thus, it is concluded tht the disruption of microtubules by MeHg resulted in the inhibition of the synthesis of tubulin itself through autoregulatory repression in post-transcriptional processes as in the case of colchicine treatment.
- 日本トキシコロジー学会の論文
著者
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Kobayashi Yuka
Department of Pharmacology, Wakayama Medical University
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Miura Kouzou
ヤクルト中央研究所
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Miura Kouzou
Central Institute For Microbiological Research
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Imura Nobumasa
Department of Public Health and Molecular Toxicology, School of Pharmaceutical Sciences, Kitasato Un
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Imura Nobumasa
Department Of Public Health And Molecular Toxicology School Of Pharmaceutical Sciences Kitasato Univ
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Toyoda Haruka
Pharmaceutical And Medical Safety Bureau Blood And Blood Products Division Ministry Of Health And We
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Kobayashi Yuka
Department Of Cardiology And Internal Medicine Yamashiro Public Hospital
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Miura Kyoko
Central Institute For Microbiological Research
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Miura Kyoko
Department Of Medicine Metabolism And Endocrinology Juntendo University School Of Medicine
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Miura Kyoko
Department Of Environmental Science Faculty Of Economics Wako University
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Imura Nobumasa
Department Of Public Health And Molecular Toxicology School Of Pharmaceutical Sciences Kitasato Univ
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Kobayashi Yuka
Department Of Public Health And Molecular Toxicology School Of Pharmaceutical Sciences Kitasato Univ
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Kobayashi Yuka
Department Of Biological And Environmental Chemistry Faculty Of Agriculture Tottori University
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