Disaccharide Composition of Glycosaminoglycan Chains in Growing Vascular Endothelial Cells in Culture after Exposure to Lead
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概要
- 論文の詳細を見る
Heparan sulfate (HS) chains bind and activate fibroblast growth factor-2 (FGF-2) depending on their microstructure. The glycosaminoglycan chains present particularly as a large HS proteoglycan perlecan in vascular endothelial cells. A heavy metal lead induces a lower response to FGF-2 by inhibition of perlecan synthesis and inhibits the proliferation of the cells. The present study was undertaken to address the question whether lead influences the formation of HS chains in vascular endothelial cells. The data indicate that lead decreases the amount of disaccharide units including hexuronic acid-N-acetylglucosamine, hexuronic acid-N-sulfated glucosamine and 2-O-sulfated hexuronic acid-N-sulfated glucosamine in HS chains accumulated in the cell layer and the conditioned medium. However, no percentage of any disaccharide unit was affected by the metal. The percentage of disaccharide units in chondroitin/dermatan sulfate was also unaffected by lead. The present data support the hypothesis that lead-induced lower response of vascular endothelial cells to endogenous FGF-2 mainly results from a decrease in perlecan molecules of extracellular matrix as a result of selective inhibition of perlecan core protein synthesis rather than a change in the HS structure.
- 公益社団法人日本薬学会の論文
- 2004-12-01
著者
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Fujiwara Yasuyuki
北陸大学 薬学部環境健康学
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Fujiwara Yasuyuki
Lab. Of Pharmaceutical Health Sciences School Of Pharmacy Aichi Gakuin Univ.
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Kaji T
Organization For Frontier Research In Preventive Pharmaceutical Sciences Hokuriku University
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Kaji Toshiyuki
Organization For Frontier Res. In Preventive Pharmaceutical Sciences Hokuriku Univ.
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Kaji Toshiyuki
Department Of Environmental Science Faculty Of Pharmaceutical Sciences Hokuriku University
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Fujiwara Yasuyuki
Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University
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Plaas A
Univ. South Florida Fl Usa
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Kaji Toshiyuki
Department Of Environmental Sciences Faculty Of Pharmaceutical Sciences Hokuriku University
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Kaji Toshiyuki
Department Of Environmental Health Faculty Of Pharmaceutical Sciences:organization For Frontier Rese
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Kaji Toshiyuki
Department Of Environmental Health Faculty Of Pharmaceutical Sciences Hokuriku University
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Fujiwara Yasuyuki
Dep. Of Environmental Health Fac. Of Pharmaceutical Sciences Hokuriku Univ.
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Fujiwara Yasuyuki
Department Of Environmental Health Faculty Of Pharmaceutical Sciences
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Fujiwara Yasuyuki
Department Of Cardiovascular Surgery Seijunkai Johoku Hospital
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Plaas Anna
Department of Internal Medicine, University of South Florida Medical School
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Plaas Anna
Department Of Internal Medicine College Of Medicine University Of South Florida
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Kaji T
北陸大学薬学部:北陸大学学術フロンティア研究組織
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