Differential Effects of Sodium Spirulan on the Secretion of Fibrinolytic Proteins from Vascular Endothelial Cells : Enhancement of Plasminogen Activator Activity

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概要

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• Sodium spirulan (Na-SP) is a sulfated polysaccharide with M_r〜220000 isolated from the blue-green alga Spirulina platensis. Na-SP influences the blood coagulation-fibrinolytic system by activation of heparin cofactor II in vitro, although it has been incompletely understood whether the polysaccharide can act on vascular endothelial cell functions. In the present study, we investigated the effects of Na-SP on the fibrinolytic activity of human coronary endothelial cells in a culture system. It was found that Na-SP enhances the activity of tissue-type and urokinase-type plasminogen activators in the conditioned medium of the cells through induction of urokinase-type plasminogen activator secretion and inhibition of plasminogen activator inhibitor type 1 (PAI-1) secretion. The inhibitory effect of Na-SP on PAI-1 secretion was maintained even when sodium ion was removed or replaced by calcium ion, while it disappeared with desulfation, indicating that metal ion is not required but the sulfate group is essential for the inhibition of endothelial PAI-1 secretion by Na-SP. The present study revealed that Na-SP not only shows a strong antithrombin effect through activation of heparin cofactor II but also exhibits a fibrinolytic property through differential effects on endothelial fibrinolytic protein secretion.

• 公益社団法人日本薬学会の論文
• 2003-10-01

著者

• Lee Jung-bum

  富山大学 大学院医学薬学研究部

• Lee Jung-bum

  Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical Uni

• Lee Jung-bum

  Faculty Of Pharmaceutical Sciences University Of Toyama

• Kaji T

  Organization For Frontier Research In Preventive Pharmaceutical Sciences Hokuriku University

• Kaji Toshiyuki

  Organization For Frontier Res. In Preventive Pharmaceutical Sciences Hokuriku Univ.

• Kaji Toshiyuki

  Department Of Environmental Science Faculty Of Pharmaceutical Sciences Hokuriku University

• Yamamoto Chika

  Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University

• SHIMADA Satomi

  Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University

• HAYASHI Toshimitsu

  Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical U

• Lee J‐b

  Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical Uni

• Nakamura Ayako

  Department Of Cardiology Tokyo Women's Medical University

• Kaji Toshiyuki

  Department Of Environmental Sciences Faculty Of Pharmaceutical Sciences Hokuriku University

• Kaji Toshiyuki

  Department Of Environmental Health Faculty Of Pharmaceutical Sciences:organization For Frontier Rese

• Kaji Toshiyuki

  Department Of Environmental Health Faculty Of Pharmaceutical Sciences Hokuriku University

• Yamamoto C

  Organization For Frontier Research In Preventive Pharmaceutical Sciences Hokuriku University

• Yamamoto Chika

  Department Of Environmental Health Faculty Of Pharmaceutical Sciences Hokuriku University

• Nakamura Ayako

  Department Of Cardiology Saiseikai Kurihashi Hospital

• Hayashi Toshimitsu

  Graduate School Of Medicine And Pharmaceutical Sciences For Res.

• Hayashi Toshimitsu

  Faculty Of Pharmaceutical Sciences University Of Toyama

• Hayashi Toshimitsu

  Graduate School Of Medicine And Pharmaceutical Science For Research University Of Toyama

• Nakamura Ayako

  Department Of Environmental Health Faculty Of Pharmaceutical Sciences Hokuriku University

• Kaji T

  北陸大学薬学部:北陸大学学術フロンティア研究組織

• Shimada Satomi

  Department Of Environmental Health Faculty Of Pharmaceutical Sciences Hokuriku University

• Hayashi Toshimitsu

  Department Of Architecture School Of Science And Engineering Kinki University

• Hirooka Takashi

  Organization For Frontier Research In Preventive Pharmaceutical Sciences Hokuriku University

• Yamamoto Chika

  Organization Of Frontier Research For Preventive Pharmaceutical Science Hokuriku University:departme

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