Proteoglycans Released from Cultured Bovine Aortic Endothelial Cell Layers by Sodium Spirulan Are Both Perlecan and Biglycan(Biochemistry/Molecular Biology)
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概要
- 論文の詳細を見る
Sodium spirulan (Na-SP) is a sulfated polysaccharide isolated from the blue-green alga Spirulina platensis. Na-SP has anticoagulant and fibrinolytic properties in vitro, including activation of heparin cofactor II, enhancement of vascular endothelial cell fibrinolytic activity and stimulation of endothelial proteoglycan (PG) release. In the present study, we investigated the types of endothelial PGs whose release is stimulated by Na-SP. Na-SP stimulated the PG release in a dose- and time-dependent manner. However, heparin, dextran sulfate and hyaluronan stimulated the release of heparan sulfate PGs rather than chondroitin/dermatan sulfate PGs, whereas the release of both types of PGs was strongly stimulated by Na-SP. Sepharose CL-6B chromatography of [^<35>S]sulfate-labeled PGs showed that PGs were partially released after partial degradation of the core proteins without a change in chain length of the glycosaminoglycan chains after Na-SP treatment. On the other hand, SDS-polyacrylamide gel electrophoresis and Western blot analysis of the PG core proteins indicated that the Na-SP-releasable PGs are both a large heparan sulfate PG, perlecan, and a small chondroitin/dermatan sulfate PG, biglycan, without change in the size of the core proteins. Taken together, these results suggest that Na-SP stimulates the endothelial release of both perlecan and biglycan with the intact structures, possibly by mechanisms different from those of heparin, dextran sulfate and hyaluronan ; a part of the PG core proteins may be degraded after Na-SP treatment. Since perlecan and biglycan have antithrombin activities, the present data support the hypothesis that Na-SP may enhance local anticoagulant activity in the liquid phase on the endothelium via stimulation of endothelial PG release.
- 社団法人日本薬学会の論文
- 2005-01-01
著者
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Lee Jung-bum
富山大学 大学院医学薬学研究部
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Lee Jung-bum
Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical Uni
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Lee Jung-bum
Faculty Of Pharmaceutical Sciences University Of Toyama
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FUJIWARA Yasuyuki
Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University
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Fujiwara Yasuyuki
北陸大学 薬学部環境健康学
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Fujiwara Yasuyuki
Lab. Of Pharmaceutical Health Sciences School Of Pharmacy Aichi Gakuin Univ.
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Kaji T
Organization For Frontier Research In Preventive Pharmaceutical Sciences Hokuriku University
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Kaji Toshiyuki
Organization For Frontier Res. In Preventive Pharmaceutical Sciences Hokuriku Univ.
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Kaji Toshiyuki
Department Of Environmental Science Faculty Of Pharmaceutical Sciences Hokuriku University
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Yamamoto Chika
Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University
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Fujiwara Yasuyuki
Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University
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SHIMADA Satomi
Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University
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HAYASHI Toshimitsu
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical U
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Lee J‐b
Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical Uni
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Kaji Toshiyuki
Department Of Environmental Sciences Faculty Of Pharmaceutical Sciences Hokuriku University
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Kaji Toshiyuki
Department Of Environmental Health Faculty Of Pharmaceutical Sciences:organization For Frontier Rese
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Kaji Toshiyuki
Department Of Environmental Health Faculty Of Pharmaceutical Sciences Hokuriku University
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Yamamoto C
Organization For Frontier Research In Preventive Pharmaceutical Sciences Hokuriku University
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Yamamoto Chika
Department Of Environmental Health Faculty Of Pharmaceutical Sciences Hokuriku University
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Sakurai Shigeru
Department Of Environmental Health Faculty Of Pharmaceutical Sciences Hokuriku University:department
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Fujiwara Yasuyuki
Dep. Of Environmental Health Fac. Of Pharmaceutical Sciences Hokuriku Univ.
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Fujiwara Yasuyuki
Department Of Environmental Health Faculty Of Pharmaceutical Sciences
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Fujiwara Yasuyuki
Department Of Cardiovascular Surgery Seijunkai Johoku Hospital
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Hayashi Toshimitsu
Graduate School Of Medicine And Pharmaceutical Sciences For Res.
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Hayashi Toshimitsu
Faculty Of Pharmaceutical Sciences University Of Toyama
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Hayashi Toshimitsu
Graduate School Of Medicine And Pharmaceutical Science For Research University Of Toyama
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Kaji T
北陸大学薬学部:北陸大学学術フロンティア研究組織
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Sakurai Saori
Department Of Biochemistry Osaka University Of Pharmaceutical Sciences
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Shimada Satomi
Department Of Environmental Health Faculty Of Pharmaceutical Sciences Hokuriku University
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Hayashi Toshimitsu
Department Of Architecture School Of Science And Engineering Kinki University
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Hirooka Takashi
Organization For Frontier Research In Preventive Pharmaceutical Sciences Hokuriku University
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Yamamoto Chika
Organization Of Frontier Research For Preventive Pharmaceutical Science Hokuriku University:departme
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