Inhibition of the Association of Proteoglycans with Cultured Vascular Endothelial Cell Layers by Calcium and Sodium Spirulan
スポンサーリンク
概要
- 論文の詳細を見る
Vascular endothelial proteoglycans exhibit an antithrombogenic activity by activating antithrombin III and heparin cofactor II on the luminal surface of the vascular wall. Calcium spirulan (Ca-SP) is a novel sulfated polysaccharide isolated from the blue-green alga Spirulina platensis. Since Ca-SP exhibits antithrombin activity by activation of heparin cofactor II, we hypothesized that the polysaccharide may influence the metabolism of anticoagulant proteoglycans synthesized by endothelial cells. When cultured bovine aortic endothelial cells were treated with Ca-SP (50 μg/ml or less) in the presence of [^<35>S]sulfate for 24 hr, the accumulation of labeled proteoglycans in the cell layer was decreased but that in the conditioned medium was significantly increased, indicating that Ca-SP inhibits the association of proteoglycans with vascular endothelial cell layers. Na-SP, which was prepared by replacement of calcium ion with sodium ion, showed an equal effect. When the endothelial cells were labeled with [^<35>S]sulfate and then treated with Ca-SP (5 μg/ml or more) for 1 hr in the absence of [^<35>S]sulfate, the percentage of [^<35>S]sulfate-labeled proteoglycans released into the medium was markedly increased by Ca-SP. DEAE-Sephacel ion exchange chromatography of [^<35>S]sulfate-labeled proteoglycans released into the medium from Na-SP-treated cells indicated that Na-SP stimulates the release of both heparan and chondroitin/dermatan sulfate proteoglycans from the cell layer. Taking these results together it is suggested that Ca-SP and Na-SP promote the release of proteoglycans from vascular endothelial cells by inhibiting the association of the macromolecules with the cell layer.
- 社団法人日本薬学会の論文
- 2002-06-01
著者
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Lee Jung-bum
富山大学 大学院医学薬学研究部
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Lee Jung-bum
Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical Uni
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Lee Jung-bum
Faculty Of Pharmaceutical Sciences University Of Toyama
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Fujiwara Yasuyuki
北陸大学 薬学部環境健康学
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Fujiwara Yasuyuki
Lab. Of Pharmaceutical Health Sciences School Of Pharmacy Aichi Gakuin Univ.
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Kaji T
Organization For Frontier Research In Preventive Pharmaceutical Sciences Hokuriku University
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Kaji Toshiyuki
Organization For Frontier Res. In Preventive Pharmaceutical Sciences Hokuriku Univ.
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Kaji Toshiyuki
Department Of Environmental Science Faculty Of Pharmaceutical Sciences Hokuriku University
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Yamamoto Chika
Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University
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Fujiwara Yasuyuki
Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University
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SHIMADA Satomi
Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University
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HAYASHI Toshimitsu
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical U
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Lee J‐b
Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical Uni
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Kaji Toshiyuki
Department Of Environmental Sciences Faculty Of Pharmaceutical Sciences Hokuriku University
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Kaji Toshiyuki
Department Of Environmental Health Faculty Of Pharmaceutical Sciences:organization For Frontier Rese
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Kaji Toshiyuki
Department Of Environmental Health Faculty Of Pharmaceutical Sciences Hokuriku University
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Yamamoto Chika
Department Of Environmental Health Faculty Of Pharmaceutical Sciences Hokuriku University
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Fujiwara Yasuyuki
Department Of Cardiovascular Surgery Seijunkai Johoku Hospital
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Hayashi Toshimitsu
Graduate School Of Medicine And Pharmaceutical Sciences For Res.
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Hayashi Toshimitsu
Faculty Of Pharmaceutical Sciences University Of Toyama
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Hayashi Toshimitsu
Graduate School Of Medicine And Pharmaceutical Science For Research University Of Toyama
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Kaji T
北陸大学薬学部:北陸大学学術フロンティア研究組織
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Sakurai Saori
Department Of Biochemistry Osaka University Of Pharmaceutical Sciences
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Shimada Satomi
Department Of Environmental Health Faculty Of Pharmaceutical Sciences Hokuriku University
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Hayashi Toshimitsu
Department Of Architecture School Of Science And Engineering Kinki University
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Hirooka Takashi
Organization For Frontier Research In Preventive Pharmaceutical Sciences Hokuriku University
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Yamamoto Chika
Organization Of Frontier Research For Preventive Pharmaceutical Science Hokuriku University:departme
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