Cloning and Expression of Calmodulin Gene in Scoparia dulcis(Pharmacognosy)
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概要
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A homology-based cloning strategy yielded a cDNA clone, designated Sd-cam, encoding calmodulin protein from Scoparia dulcis. The restriction digests of genomic DNA of S. dulcis showed a single hybridized signal when probed with the fragment of this gene in Southern blot analyses, suggesting that Sd-cam occurs as a sole gene encoding calmodulin in the plant. The reverse-transcription polymerase chain reaction analysis revealed that Sd-cam was appreciably expressed in leaf, root and stem tissues. It appeared that transcription of this gene increased transiently when the leaf cultures of S. dulcis were treated with methyl jasmonate and calcium ionophore A23187. These results suggest that transcriptional activation of Sd-cam is one of the early cellular events of the methyl jasmonate-induced responses of S. dulcis.
- 2007-06-01
著者
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Lee Jung-bum
富山大学 大学院医学薬学研究部
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Lee Jung-bum
Faculty Of Pharmaceutical Sciences University Of Toyama
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Lee J‐b
Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical Uni
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SUGIYAMA Ryuji
Faculty of Pharmaceutical Sciences, University of Toyama
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HAYASHI Toshimitsu
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
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KUROSAKI Fumiya
Faculty of Pharmaceutical Sciences, University of Toyama
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Hayashi Toshimitsu
Graduate School Of Medicine And Pharmaceutical Sciences For Res.
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Hayashi Toshimitsu
Faculty Of Pharmaceutical Sciences University Of Toyama
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Hayashi Toshimitsu
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Hayashi Toshimitsu
Graduate School Of Medicine And Pharmaceutical Science For Research University Of Toyama
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Hayashi T
Faculty Of Pharmaceutical Sciences Josai University
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Sugiyama Ryuji
Faculty Of Pharmaceutical Sciences University Of Toyama
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Shite Masato
Laboratory Of Plant Resource Sciences Graduate School Of Medicine And Pharmaceutical Science For Res
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Hirooka Takashi
Organization For Frontier Research In Preventive Pharmaceutical Sciences Hokuriku University
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SAITOH Daisuke
Faculty of Pharmaceutical Sciences, University of Toyama
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ASAKURA Yuki
Faculty of Pharmaceutical Sciences, University of Toyama
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NKEMBO Marguerite
Faculty of Pharmaceutical Sciences, University of Toyama
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SHITE Masato
Faculty of Pharmaceutical Sciences, University of Toyama
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Asakura Yuki
Faculty Of Pharmaceutical Sciences University Of Toyama
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Kurosaki Fumiya
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Saitoh Daisuke
Faculty Of Pharmaceutical Sciences University Of Toyama
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Kurosaki Fumiya
Laboratory Of Plant Resource Sciences Graduate School Of Medicine And Pharmaceutical Science For Res
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Nkembo Marguerite
Faculty Of Pharmaceutical Sciences University Of Toyama
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