クロトリマゾール耐性の付与による清酒酵母の発酵力改善

概要

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Mutants resistant to clotrimazole (CTZ) were isolated from a non-urea forming sake yeast strain, HL-163,and an isoamyl acetate highly producing strain, Ffe-13. Most of the CTZ-resistant mutants fermented sake mash faster, and produced higher amounts of alcohol than their parent strains, while keeping their parental advantages for sake making. CTZ-resistant mutants were also resistant to cycloheximide, sulfometuron methyl, cerulenin, chloramphenicol, and oligomycin. Since the chemical structures of these drugs and their actions toward yeast cell are widely different from each other, CTZ-resistant mutants showed pleiotropic drug resistance (PDR) phenotypes. In a Northern analysis, the transcript of PDR5,a gene encoding an ATP-binding cassette (ABC) transporter, which extrudes the drugs out of yeast cell, was overexpressed in CTZ-resistant mutants. These observations indicated that CTZ-resistant mutants with improved fermentative activity must have a certain mutation related to PDR.
社団法人日本生物工学会の論文
1998-05-25