Improved Transformation of the Halo-Tolerant Yeast Zygosaccharomyces rouxii by Electroporation
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概要
- 論文の詳細を見る
To improve the transformation efficiency of Zygosaccharomyces rouxii by electroporation, glycerol was added to the electroporation buffer and the cells were frozen at −80 °C. These alterations drastically increased transformation efficiency, and we found that competent cells can be preserved at −80 °C without decreasing their transformation efficiency for at least 30 d.
- 2010-05-23
著者
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Suzuki Kazushi
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Suzuki Kazushi
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
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WATANABE Jun
Manufacturing Division, Yamasa Corporation
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UEHARA Kenji
Manufacturing Division, Yamasa Corporation
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MOGI Yoshinobu
Manufacturing Division, Yamasa Corporation
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Yamazaki Tatsuo
Manufacturing Division Yamasa Corporation
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Uehara Kenji
Manufacturing Division Yamasa Corporation
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Watanabe Takeshi
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Mogi Yoshinobu
Manufacturing Division Yamasa Corporation
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Watanabe Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Watanabe Jun
Manufacturing Division Yamasa Corporation:department Of Applied Biological Chemistry Faculty Of Agri
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Suzuki Kazushi
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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