Measurement for Canine IgE Using Canine Recombinant High Affinity IgE Receptor α Chain (FcεRIα)
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概要
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To detect allergen-specific IgE in dogs with allergic diseases, we developed a recombinant canine high affinity IgE receptor α chain (FcεRIα)-based IgE detection system. Using the recombinant protein of canine FcεRIα expressed by an Escherichia coli expression system, we could detect house dust mite (Dermatophagoides farinae) allergen-specific IgE in sera from dogs naturally and experimentally sensitized to this allergen with ELISA and western blotting. The IgE binding activity of recombinant canine FcεRIα on ELISA was impaired by heat treatment of these sera. The specificity of this recombinant canine FcεRIα-based IgE detection system was confirmed by inhibition assays with canine IgE. The recombinant canine FcεRIα-based IgE detection system established in this study offers an alternative tool to measure allergen-specific IgE in dogs.
著者
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KURATA Keigo
Institute of Tokyo Environmental Allergy
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Ohmori Keitaro
Laboratory of Veterinary Molecular Pathology and Therapeutics, Division of Animal Life Science, Grad
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Masuda Kenichi
Animal Allergy Clinical Lab. Inc. Kanagawa
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Tsujimoto Hajime
Department Of Internal Medicine And Veterinary Hospital Division Of Veterinary Medicine The Universi
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Sakaguchi Masahiro
Department Of Anesthesiology Kyoto Prefectural University Of Medicine
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Maeda Sadatoshi
Department Of Veterinary Internal Medicine Graduate School Of Agricultural And Life Sciences The Uni
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Tsukui Toshihiro
Nippon Zenyaku Kogyo Co. Ltd.
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KURATA Keigo
Institute of Tokyo Environmental Allergy, 2–2–4 Yushima, Bunkyo-ku, Tokyo 113–0034, Japan
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IWABUCHI Shigehiro
Nippon Zenyaku Kogyo Co., Ltd., 1–1 Tairanoue Sasagawa, Asaka-machi, Koriyama, Fukushima 963–0196, Japan
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IWABUCHI Shigehiro
Nippon Zenyaku Kogyo Co., Ltd., 1–1 Tairanoue Sasagawa, Asaka-machi, Koriyama, Fukushima 963–0196, Japan
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TSUJIMOTO Hajime
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
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TSUKUI Toshihiro
Nippon Zenyaku Kogyo Co., Ltd., 1–1 Tairanoue Sasagawa, Asaka-machi, Koriyama, Fukushima 963–0196, Japan
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OHMORI Keitaro
Laboratory of Veterinary Molecular Pathology and Therapeutics, Division of Animal Life Science, Graduate School, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology, 3–5–8 Saiwai-cho, Fuchu, Tokyo 183&
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MASUDA Kenichi
Animal Allergy Clinical Laboratories, Inc., 5–4–30 Nishihashimoto, Midori-ku, Sagamihara Kanagawa 252–0131, Japan
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