Flow Cytometric Analysis of Lymphocyte Proliferative Responses to Food Allergens in Dogs with Food Allergy

スポンサーリンク

概要

• 論文の詳細を見る

• Two different allergy tests, antigen-specific immunoglobulin E quantification (IgE test) and flow cytometric analysis of antigen-specific proliferation of peripheral lymphocytes (lymphocyte proliferation test), were performed to examine differences in allergic reactions to food allergens in dogs with food allergy (FA). Thirteen dogs were diagnosed as FA based on clinical findings and elimination diet trials. Seven dogs clinically diagnosed with canine atopic dermatitis (CAD) were used as a disease control group, and 5 healthy dogs were used as a negative control group. In the FA group, 19 and 33 allergen reactions were identified using the serum IgE test and the lymphocyte proliferation test, respectively. Likewise, in the CAD group, 12 and 6 allergen reactions and in the healthy dogs 3 and 0 allergen reactions were identified by each test, respectively. A significant difference was found between FA and healthy dogs in terms of positive allergen detection by the lymphocyte proliferation test, suggesting that the test can be useful to differentiate FA from healthy dogs but not from CAD. Both tests were repeated in 6 of the dogs with FA after a 1.5- to 5-month elimination diet trial. The IgE concentrations in 9 of 11 of the positive reactions decreased by 20–80%, whereas all the positive reactions in the lymphocyte proliferation test decreased to nearly zero (P<0.05), suggesting that lymphocytes against food allergens may be involved in the pathogenesis of canine FA.

著者

• Masuda Kenichi

  Animal Allergy Clinical Lab. Inc. Kanagawa

• FUJIMURA Masato

  Fujimura Animal Hospital

• HAYASHIYA Makio

  Hayashiya Animal Hospital

• OKAYAMA Taro

  Animal Allergy Clinical Laboratories

関連論文

• Cultivation and Characterization of Canine Skin-Derived Mast Cells
• The Antibody against Human CD25, ACT-1, Recognizes Canine T-lymphocytes in the G2/M and G0/G1 Phases of the Cell Cycle during Proliferation(Internal Medicine)
• Flow Cytometric Analysis of Lymphocyte Proliferative Responses to Food Allergens in Dogs with Food Allergy
• Clinical Efficacy of a Novel Elimination Diet Composed of a Mixture of Amino Acids and Potatoes in Dogs with Non-Seasonal Pruritic Dermatitis
• Measurement for Canine IgE Using Canine Recombinant High Affinity IgE Receptor α Chain (FcεRIα)
• Lymphocyte Blastogenic Responses to Food Antigens in Cats Showing Clinical Symptoms of Food Hypersensitivity

スポンサーリンク