Cultivation and Characterization of Canine Skin-Derived Mast Cells
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概要
- 論文の詳細を見る
It is essential to develop a technique to culture purified skin-derived mast cells (SMCs) to facilitate immunological research on allergic diseases in dogs. This study was performed to develop an efficient culture system for canine SMCs and to characterize the cells in comparison to canine bone marrow-derived mast cells (BMMCs). Enzymatically digested skin biopsy samples were cultivated in serum-free AIM-V medium supplemented with recombinant canine stem cell factor. Three to five weeks after the initiation of culture, mast cells were collected by a magnetic activated cell separation system using anti-c-Kit antibody. The collected cells were composed of a uniform population showing morphological characteristics of mast cells with a round or oval nucleus and abundant toluidine blue-positive metachromatic granules in the cytoplasm. Results of the flow cytometric analysis for the presence of cell membrane c-Kit and Fc epsilon receptor I (FcεRI) supported that approximately 90% of the cells were mast cells. The cytoplasmic granules were positive for both tryptase and chymase. Apparent dose-dependent degranulation was induced by antibody-mediated cross-linking of immunoglobulin E (IgE) bound to the cells. These cytological and immunological characteristics observed in SMCs were mostly similar to those in BMMCs; however, IgE-mediated degranulation was significantly lower in SMCs than BMMCs. The culture system of canine SMCs developed in this study would be useful to understand the pathophysiology and to develop anti-allergic therapeutics in canine allergic dermatitis.
著者
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TSUJIMOTO Hajime
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The U
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Kawarai Shinpei
First Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University
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Masuda Kenichi
Animal Allergy Clinical Laboratories
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Ohmori Keitaro
Laboratory of Veterinary Molecular Pathology and Therapeutics, Division of Animal Life Science, Grad
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MATSUURA Shinobu
Moores UCSD Cancer Center
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YASUDA Nobutaka
Animal Allergy Clinical Laboratories
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NAGATA Masahiko
Animal Dermatology Center
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SAKAGUCHI Masahiro
First Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University
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Masuda Kenichi
Animal Allergy Clinical Laboratories Inc.
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Masuda Kenichi
Animal Allergy Clinical Lab. Inc. Kanagawa
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Tsujimoto Hajime
Department Of Internal Medicine And Veterinary Hospital Division Of Veterinary Medicine The Universi
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Yasuda Nobutaka
Animal Allergy Clinical Lab. Inc. Kanagawa
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