Identification of Bovine Serum Albumin as an IgE-Reactive Beef Component in a Dog with Food Hypersensitivity against Beef
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概要
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IgE-reactive beef components were examined by an immunoblot analysis using a serum from a dog with food hypersensitivity against beef. The immunoblot analysis revealed a distinct band at approximately 66 kDa and a faint band at approximately 50 kDa. The immunoblot analysis for serum IgE reactivity to bovine serum albumin (BSA) also revealed a positive band at 66 kDa. Serum IgE reactivity to the 66-kDa protein of beef was diminished by pre-incubating the serum sample with BSA. Furthermore, a positive reaction to BSA was detected in intradermal testing in the dog. These results clearly indicated that BSA was an IgE-reactive beef component in the dog with food hypersensitivity against beef.
著者
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Tsujimoto Hajime
Department Of Internal Medicine And Veterinary Hospital Division Of Veterinary Medicine The Universi
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Sakaguchi Masahiro
Department Of Anesthesiology Kyoto Prefectural University Of Medicine
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KAWARAI Shinpei
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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OHMORI Keitaro
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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MASUDA Kenichi
Laboratory for Vaccine Design, Research Center for Allergy and Immunology, Yokohama Institute, RIKEN
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YASUDA Nobutaka
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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SAKAGUCHI Masahiro
Department of Otolaryngology Head and Neck Surgery, Graduate School of Medicine, Chiba University
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