Effect of Synthetic Cell-Penetrating Peptides on TrkA Activity in PC12 Cells

スポンサーリンク

概要

• 論文の詳細を見る

• As TrkA, a high-affinity receptor of nerve growth factor (NGF), is a potential target for relieving uncontrolled inflammatory pain, an effective inhibitor of TrkA has been required for pain management. To identify a specific inhibitor of TrkA activity, we designed cell-penetrating peptides combined with amino-acid sequences in the activation loop of TrkA to antagonize tyrosine kinase activity. To select a peptide inhibiting TrkA activity, we examined the effect of cell-penetrating peptides on tyrosine kinase activity of recombinant TrkA in vitro and studied their effects on NGF-stimulated neurite outgrowth and protein phosphorylation in PC12 cells. Thereafter we investigated the effect of the selected peptide on NGF-stimulated TrkA activity and the expression of transient receptor potential channel 1 in PC12 cells. The selected peptide inhibited TrkA activity, but did not inhibit tyrosine kinase activities of other receptor-type tyrosine kinases in vitro. It also suppressed NGF-stimulated responses in PC12 cells. The selected synthetic cell-penetrating peptide antagonizing TrkA function would be a candidate for inflammatory pain therapy.

• 社団法人 日本薬理学会の論文
• 2008-01-20

著者

• SAKAGUCHI Masahiro

  Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University

• Murata Eri

  Department of Anesthesiology and Reanimatology, University of Fukui, Faculty of Medical Sciences, Ja

• Ueda Koyo

  Department of Anesthesiology and Reanimatology, University of Fukui, Faculty of Medical Sciences, Ja

• Kuroda Yoshihiro

  Department of Pharmaceutical Health Care, Faculty of Pharmaceutical Sciences, Himeji Dokkyo Universi

• Abe Mineo

  Graduate School of Pharmaceutical Sciences, Kyoto University, Japan

• Shigemi Kenji

  Department of Anesthesiology and Reanimatology, University of Fukui, Faculty of Medical Sciences, Ja

• Hirose Munetaka

  Department of Anesthesiology and Reanimatology, University of Fukui, Faculty of Medical Sciences, Ja

• TANAKA Yoshifumi

  Department of Anesthesiology, Graduate School of Medical Science, Kyoto Prefectural University of Me

• Tanaka Yoshifumi

  Department Of Anesthesiology Kyoto Prefectural University Of Medicine

• Abe Mineo

  Graduate School Of Pharmaceutical Sciences Kyoto University

• Shigemi Kenji

  Department Of Anesthesiology And Reanimatology Faculty Of Medical Sciences Fukui University

• Kuroda Yoshihiro

  Department Of Pharmaceutical Health Care Faculty Of Pharmaceutical Sciences Himeji Dokkyo University

• HIRATA MASASHI

  Department of Agricultural Chemistry, Faculty of Bioresources, Mie University

• Hirata Masashi

  Department Of Anesthesiology Kyoto Prefectural University Of Medicine

• Hirata Masashi

  Department Of Agricultural Chemistry Faculty Of Bioresources Mie University

• Sakaguchi Masahiro

  Department Of Anesthesiology Kyoto Prefectural University Of Medicine

• TAKATORI Mayumi

  Department of Anesthesiology, Kyoto Prefectural University of Medicine

• ISADA Tetsuro

  Department of Anesthesiology and Reanimatology, Faculty of Medical Sciences, Fukui University

• SHIBAZAKI Masayuki

  Department of Anesthesiology, Kyoto Prefectural University of Medicine

• SHIMIZU Fumihiro

  Department of Anesthesiology, Kyoto Prefectural University of Medicine

• FUKAZAWA Keita

  Department of Anesthesiology, Kyoto Prefectural University of Medicine

• KAGEYAMA Kyoko

  Department of Anesthesiology, Kyoto Prefectural University of Medicine

• Isada Tetsuro

  Department Of Anesthesiology And Reanimatology Faculty Of Medical Sciences Fukui University

• Kageyama Kyoko

  Department Of Anesthesiology Kyoto Prefectural University Of Medicine

• Kageyama Kyoko

  Department Of Anesthesiology Kyoto Prefectual University Of Medicine

• Fukazawa Keita

  Department Of Anesthesiology Kyoto Prefectural University Of Medicine

• Murata Eri

  Department Of Anesthesiology And Reanimatology Faculty Of Medical Sciences University Of Fukui

• Ueda Koyo

  Department Of Anesthesiology And Reanimatology Faculty Of Medical Sciences University Of Fukui

• Takatori Mayumi

  Department Of Anesthesiology Kyoto Prefectural University Of Medicine

• Shimizu Fumihiro

  Department Of Anesthesiology Kyoto Prefectural University Of Medicine

• Shibazaki Masayuki

  Department Of Anesthesiology Kyoto Prefectural University Of Medicine

• Hirose Munetaka

  Department Of Anesthesiology And Reanimatology Faculty Of Medical Sciences University Of Fukui

• Tanaka Yoshifumi

  Department Of Anesthesia Faculty Of Medicine University Of Tokyo

• HIROSE Munetaka

  Department of Anesthesia and Pain Clinic, Kusatsu General Hospital

関連論文

• Potential Immunological Adjuvant of K-Type CpG-Oligodeoxynucleotides Enhanced the Cell Proliferation and IL-6 mRNA Transcription in Canine B cells
• Spin and Charge Susceptibilities in the d-p Model
• A Synthetic Cell-Penetrating Peptide Antagonizing TrkA Function Suppresses Neuropathic Pain in Mice
• Magnetic and Electrical Properties of Nonequilibrium Fe-W Alloys Produced by Sputter Deposition
• A portable blood analyzer that uses on-line data management to deliver higher-quality patient information
• Isolation and Characterization of cDNAs That Encode Homologs of a Pathogenesis-related Protein Allergen from Cryptomeria japonica(Biochemistry & Molecular Biology)
• A subcutaneously injected UV-inactivated SARS coronavirus vaccine elicits systemic humoral immunity in mice
• Report on the computer software contest at the 43rd Congress of the Japan Society of Anesthesiology
• Report on the computer software contest at the 42nd Congress of the Japan Society of Anesthesiology
• Comments on Superconducting Fluctuation and Transport Phenomena in a Quasi-2D Electronic System (Condensed Matter: Electronic Structure, Electrical, Magnetic and Optical Properties)
• Comments on Superconducting Fluctuation and Transport Phenomena in a Quasi-2D Electronic System
• Phase Diagram of the Electron-Doped Cuprate Superconductors
• Pseudogap Phenomena and Phase Diagram in the Two-Band Hubbard Model : Condensed Matter: Electronic Properties, etc.
• Fermi Liquid and Non-Fermi Liquid in Anderson Lattice : Condensed Matter: Electronic Properties, etc.
• Critical Study on 2D Superconducting Fluctuation : in a Weak Coupling Case : Condensed Matter: Electronic Properties, etc.
• Quadrupolar Order, Hidden Octupolar Order and Tiny Magnetic Moment in URu_2Si_2 : Condensed Matter: Electronic Properties, etc.
• Pseudogap Induced by Superconducting Fluctuation in the d-p Model
• Non-Fermi Liquid in Multichannel Degenerate Anderson Lattice
• Fermi Liquid in Degenerate Anderson Lattice
• Fermi Liquid and Non-Fermi Liquid in M-Channel N Fold Degenerate Anderson Lattice
• Superexchage Interaction in the d-p Model
• Superconductivity in the d-p Model : Effects of Quasi-2D Fluctuation
• Infrared Divergence and Non-Fermi Liquid in Multichannel Degenerate Anderson Model. II.
• d-Site Substitution Effects in the d-p Model
• Infrared Divergence and Non-Fermi Liquid in Multichannel Degenerate Anderson Model
• Pairing Interaction and Superconducting Transition Temperature in the d-p Model
• Infrared Divergence in Pseudo-Particle Spectra
• Annual Variations of the Elemental Concentrations of PM_ in Ambient Air of Nagoya City as Determined by ICP-AES and ICP-MS
• Chemical Characterization of Airborne Particulate Matter in Ambient Air of Nagoya, Japan, as Studied by the Multielement Determination with ICP-AES and ICP-MS
• Lead Isotopic Compositions of Atmospheric Suspended Particulate Matter in Nagoya City as Measured by HR-ICP-MS
• Magnetic Support from Partially Deacetylated Chitin for Enzyme Immobilization
• Differential Expression of Prolactin Releasing Peptide and Tyrosine Hydroxylase in Fibers Derived from the Same Neurons in the Rat
• Immunological Findings in 3 Dogs Clinically Diagnosed with Allergic Rhinitis
• Foot Hyperalgesia after Thoracic Burn Injury : Histochemical, Behavioral and Pharmacological Studies
• Superconductor-Insulator Transition in the d-p Model
• Local Administration of a Synthetic Cell-Penetrating Peptide Antagonizing TrkA Function Suppresses Inflammatory Pain in Rats
• Significance of Heterochronic Differences in Neural Crest Cell Migration and Sclerotomal Development in Evolution of the Trunk Body Organization from Agnathans to Gnathostomes(Developmental Biology)
• Migration and Colonization Patterns of HNK-1-Immunoreactive Neural Crest Cells in Lamprey and Swordtail Embryos
• Non-IgE, -IgG4 Antibody to Japanese Cedar Pollen Allergens : Comparison of Its Prevalence and Titers between Pollinosis Patients and Non-Patients
• Comments on the Mori Formula for Transport Coefficients II
• Comments on the Mori Formula for Transport Coefficients
• Obesity as a risk factor for GERD in Japan
• Effect of Synthetic Cell-Penetrating Peptides on TrkA Activity in PC12 Cells
• Hemorrhagic shock 3 days after catheterization from the axillary vein
• Five-day pain management regimen using patient-controlled analgesia facilitates early ambulation after cardiac surgery
• Genetic diversity and kinetic properties of Trypanosoma cruzi dihydroorotate dehydrogenase isoforms
• Relationship between Airborne Cry j 1 and the Onset Time of the Symptoms of Japanese Cedar Pollinosis Patients
• MyD88 but Not TLR2, 4 or 9 Is Essential for IL-12 Induction by Lactic Acid Bacteria
• On the Strong-Coupling Effects in Superfluid He^3
• Physiological Changes during a Holiday Tour
• Repeated Exposures to Hyperbaric Air Suppress Neurite Outgrowth in PC12 Cells
• Cisplatin-based chemotherapy followed by surgery for malignant nonseminomatous germ cell tumor of mediastinum : one institution's experience
• Successful video-thoracoscopic drainage for descending necrotizing mediastinitis
• Current problems in the diagnosis and treatment of hospital-acquired methicillin-resistant Staphylococcus aureus pneumonia
• Histochemical Expression of Inducible Nitric Oxide Synthase and Inflammatory Cytokines in Alveolar Macrophages in Patients with ARDS
• Charge-Fluctuation-Mediated Superconductivity in the d-p Model : Critical Study of the Retardation Effects
• The Ingap State and Superconductivity in the Cuprate Oxide Compounds : IV-A Electronic States : IV Mechanisms of Superconductivity; Theory
• Electronic States in the Cu-Oxide Superconductors
• Charge Fluctuation and Superconductivity in the d-p Model
• Charge Fluctuation in the d-p Model : Effects of the Repulsive Interaction between d-and p-Holes
• Impurity Effects in Lattice Anderson Model
• Finite Temperature Crossover in the d-p Model
• Kondo Insulator and Charge Transfer Insulator in Lattice Anderson Model
• EFFECT OF NICARDIPINE HYDROCHLORIDE ON CIRCULATING BLOOD VOLUME AND VASCULAR COMPLIANCE IN DOGS
• Ontogeny of respiratory area of a marine teleost, porgy, Pagrus major
• MyD88 but Not TLR2, 4 or 9 Is Essential for IL-12 Induction by Lactic Acid Bacteria
• Ring-Opening Polymerization of 1,1,2,2-Tetramethyl-1,2-disilacyclopentane via Palladium Complex-Catalyzed Si-Si Bond Metathesis
• Local Administration of a Synthetic Cell-Penetrating Peptide Antagonizing TrkA Function Suppresses Inflammatory Pain in Rats
• Toborinone and olprinone, phosphodiesterase III inhibitors, inhibit human platelet aggregation due to the inhibition of both calcium release from intracellular stores and calcium entry
• A basic policy for allergen standerdization in our country and standardization of Japanese cedar (Cryptomeria japonica) pollen extracts
• Superconductor-Insulator Transition in the d-p Model
• Effect of pollen exposure on serum IgE and IgG antibody responses in Japanese cedar pollinosis patients
• Specificity of an enzyme-linked immunosorbent assay for dog IgE antibody to Japanese cedar (Cryptomeria japonica) pollen
• Electronic State of the Anderson Lattice over the Whole Temperature Range
• Transport Coefficients for Many Electron System : Comparative Study of Mori Formula and Kubo Formula
• Effect of Antiferromagnetic Spin-Fluctuation on the Electronic States in the d-p Model
• 1/N-Expansion Study of a U-Finite Degenerate Anderson Model
• Electronic States in High-T_c Copper-Oxide Superconductors
• Comments on Surface-Induced Magnetism of Liquid ^3He in Restricted Geometries
• Velocity Correlation Function of Electron Interacting with Impurity Centers in Two Dimensions
• Anderson Localization and Energy Gap in Two-Dimensional Superconductors
• Paramagnon and the BCS State in He^3 : Effect of a Magnetic Field
• Anderson Localization and Ginzburg-Landau Equations in Two-Dimensional Superconductors
• Theory of the Surface-Induced Magnetism in Liquid ^3He
• Relationship between numbers of birch pollen and different particle sizes of the pollen antigens (Bet v) in the air in Stockholm, Sweden
• Diagnostic time in total colonoscopy shortened using special test meals
• Transcutaneous pressure at which the internal jugular vein is collapsed on ultrasonic imaging predicts easiness of the venous puncture
• Current practice of preoperative fasting : a nationwide survey in Japanese anesthesia-teaching hospitals
• Interaction between cFLIP_L and Itch, a ubiquitin ligase, is obstructed in Trypanosoma cruzi-infected human cells
• C-arm fluoroscopic cone-beam CT for guidance of chemical thoracic sympathectomy
• Identification of Bovine Serum Albumin as an IgE-Reactive Beef Component in a Dog with Food Hypersensitivity against Beef
• An Origin of an Anomalously Narrow Energy-Band in a Dense Kondo System : Progress Letters
• A Synthetic Cell-Penetrating Peptide Antagonizing TrkA Function Suppresses Neuropathic Pain in Mice
• Persistent upper airway obstruction after aneurysmectomy of brachiocephalic trunk
• Cardiotoxic Local Anesthetics Increasingly Interact with Biomimetic Membranes under Ischemia-Like Acidic Conditions
• Measurement for Canine IgE Using Canine Recombinant High Affinity IgE Receptor α Chain (FcεRIα)
• Effects of TrkA inhibitory peptide on cancer-induced pain in a mouse melanoma model
• Superconductivity in the Dense Kondo System : Condensed Matter and Statistical Physics
• A Retrospective Study on Adverse Reactions to Canine Vaccines in Japan.
• Antigen-Specific Histamine Release in Dogs with Food Hypersensitivity.

もっと見る 閉じる

スポンサーリンク