Potential Immunological Adjuvant of K-Type CpG-Oligodeoxynucleotides Enhanced the Cell Proliferation and IL-6 mRNA Transcription in Canine B cells
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概要
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CpG oligodeoxynucleotides (CpG-ODNs) are ligands for toll-like receptor 9 (TLR9), signaling of which plays a role in innate immunity by inducing T helper 1 (TH1)-cell responses and pro-inflammatory cytokine production. The activation of TLR9 signaling is considered to be effective for the therapy of cancer, infectious diseases, and allergies and preclinical studies using CpG-ODNs have been performed in dogs and humans. In order to investigate the precise mechanisms responsible for the effect of CpG-ODNs in dogs, we examined their role in cell proliferation and cytokine gene expression in canine B cells. Canine B cells were collected by a magnetic cell isolation method using anti-CD21 antibody. Flow cytometric analysis for the intracellular CD79α revealed the purity of canine B cells to be as high as 90.2 ± 2.1%. Transcription of TLR2, TLR4, and TLR9 mRNA on canine CD21+ cells was confirmed by reverse-transcript polymerase chain reaction (RT-PCR). CpG-ODNs induced dose-dependent proliferation of canine CD21+ cells (P<0.05 compared with control-ODNs) detected by BrdU incorporation. Quantification of IL-6, IL-10, and IL-12p40 mRNA transcription on canine CD21+ cells revealed that CpG-ODNs enhanced IL-6 mRNA transcription but not IL-10 and IL-12p40 mRNA transcription (P<0.05 compared with control-ODNs). These responses to CpG-ODNs in the canine B cells indicated that CpG-ODNs would be useful as an immunological adjuvant for vaccine in dogs.
著者
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SATO Kaoru
Department of Earth and Planetary Science, The University of Tokyo
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TSUJIMOTO Hajime
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The U
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KAWARAI Shinpei
Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University
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HORIGUCHI Anna
Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University
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KURATA Keigo
Institute of Tokyo Environmental Allergy
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KIUCHI Akio
Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University
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SAKAGUCHI Masahiro
Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University
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Tsujimoto Hajime
Department Of Internal Medicine And Veterinary Hospital Division Of Veterinary Medicine The Universi
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Sakaguchi Masahiro
Department Of Anesthesiology Kyoto Prefectural University Of Medicine
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Kiuchi Akio
Department Of Veterinary Microbiology Azabu University
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SATO KAORU
Department of Anesthesiology, Fukushima Medical University School of Medicine
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KAWARAI Shinpei
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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