Verification of Measurement of the Feline Serum Amyloid A (SAA) Concentration by Human SAA Turbidimetric Immunoassay and Its Clinical Application(Clinical Pathology)
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概要
- 論文の詳細を見る
Serum amyloid A (SAA) is one of the major acute phase proteins in cats that has potential to be used as an inflammatory marker. A previous study showed that the human SAA turbidimetric immunoassay (hSAA-TIA) could be used to measure the SAA concentration in cats. The objectives of the present study were to assess use of hSAA-TIA for determining the feline SAA concentration and to evaluate its clinical application. Recombinant feline SAA protein (rfSAA) was expressed in Escherichia coli and purified for SDS-PAGE and immunoblot analysis with anti-human SAA antibodies. The concentration of rfSAA was determined by ELISA and hSAA-TIA. Plasma SAA concentrations were measured in healthy and diseased cats by hSAA-TIA. The time-courses changes in the SAA and α1-acid glycoprotein (AGP) concentrations in the cats after ovariohysterectomy were investigated. In SDS-PAGE, rfSAA was detected as a clear band that reacted with anti-human SAA antibodies. There was significant correlation between the SAA concentration measured by ELISA and hSAA-TIA. The SAA concentration of the diseased cats (n=263) was significantly increased (P<0.01; 0.0-88.9mg/l, mean: 7.52mg/l) compared with that in the healthy cats (n=26; 0.0-0.9mg/l, mean: 0.14mg/l). No correlation was observed between SAA and WBC in the diseased cats. The SAA concentration changed more rapidly and remarkably than the AGP concentration after ovariohysterectomy. The present study revealed that hSAA-TIA is useful for determination of the feline SAA concentration. Measurement of the SAA concentration, in addition to the WBC count, would be clinically valuable as a routine test to detect inflammation.
- 社団法人日本獣医学会の論文
- 2008-11-25
著者
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TSUJIMOTO Hajime
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The U
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OHNO Koichi
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The U
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Tsujimoto Hajime
Department Of Veterinary Internal Medicine Graduate School Of Agricultural And Life Sciences The Uni
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Goto-Koshino Yuko
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The U
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Ohno Koichi
Department Of Veterinary Internal Medicine Graduate School Of Agricultural And Life Sciences The Uni
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TAMAMOTO Takashi
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The U
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OHMI Aki
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The U
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Ohno Koichi
Department Of Veterinary Internal Medicine The University Of Tokyo
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Ohno Koichi
Department Of Chemical Engineering Faculty Of Engineering Niigata University
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Tsujimoto Hajime
Univ. Tokyo Tokyo Jpn
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Tsujimoto Hajime
Department Of Internal Medicine And Veterinary Hospital Division Of Veterinary Medicine The Universi
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Tamamoto Takashi
Department Of Veterinary Internal Medicine Graduate School Of Agricultural And Life Sciences The Uni
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Goto-Koshino Yuko
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Ohmi Aki
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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OHMI Aki
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Science, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
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