A Rapid and Simple Method to Obtain Canine Peripheral Blood-Derived Macrophages
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概要
- 論文の詳細を見る
Macrophages play an important role in a variety of situations, including pathogen elimination, inflammation, and tissue repair. However, these cells are not fully studied in dogs, in part, due to the difficulty of efficiently isolating and culturing them in vitro. In this study, we cultured canine peripheral blood mononuclear cells (PBMCs) with 10 ng/ml of phorbol 12-myristate-13-acetate (PMA) for 5 days to obtain macrophages. A high number of round-adherent cells were obtained without the addition of any cytokine. These cells showed active phagocytic activity and a cell surface antigen profile different from dendritic cells. Our method facilitates a high yield of macrophages in a short cultivation period compared with previous studies. This method might be a powerful tool to study macrophage functions in dogs.
著者
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TSUJIMOTO Hajime
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The U
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OHNO Koichi
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The U
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Goto-Koshino Yuko
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The U
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MOCHIZUKI Hiroyuki
Department of Pediatrics and Developmental Medicine, Gunma University Graduate School
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NAKAJIMA Mayumi
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The U
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KANEMOTO Hideyuki
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The U
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Ohno Koichi
Department Of Chemical Engineering Faculty Of Engineering Niigata University
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Tsujimoto Hajime
Department Of Internal Medicine And Veterinary Hospital Division Of Veterinary Medicine The Universi
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Mochizuki Hiroyuki
Department Of Pediatrics And Developmental Medicine Gunma University Graduate School Of Medicine
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Nakajima Mayumi
Department Of Pharmacology Osaka College Of Pharmacy
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Nakajima Mayumi
Department Of Veterinary Internal Medicine Graduate School Of Agricultural And Life Sciences The Uni
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MOCHIZUKI Hiroyuki
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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NAKAJIMA Mayumi
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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