Investigation of Various Methods for the Cryopreservation of Canine Bone Marrow-Derived CD34^+ Cells(Internal Medicine)
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概要
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Optimal condition for the cryopreservation of canine CD34^+ cells was explored. Canine bone marrow CD34^+ cells were isolated from 5 healthy dogs by a magnetic-activated cell-sorting system using a monoclonal antibody specific to canine CD34. These cells were cryopreserved by 4 different methods: 2 different cryoprotectant solutions-solutions A (fetal bovine serum containing 10% dimethylsulfoxide (DMSO) and B (physiological saline containing 5% DMSO, 6% hydroxyethyl starch, and 4% bovine serum albumin)-were used in combination with 2 different freezing procedures-in a rate-controlled programmed freezer (PF) and in an ordinary freezing container. The cell viability, cell recovery rates, and colony-forming unit (CFU) recovery rates were examined following cryopreservation for 1 week, 4 weeks, and 6 months. The values of these parameters were significantly higher for the CD34^+ cells that had been frozen in Solution B than for those that had been frozen in Solution A, regardless of the freezing procedure employed. The highest CFU recovery rate following cryopreservation for 6 months corresponded to the cells that had been cryopreserved with Solution B and frozen in a PF. In conclusion, cryopreservation with Solution B in a PF proved to be the most efficient of the 4 cryopreservation procedures investigated in terms of maintaining the quality of canine bone marrow-derived CD34^+ cells. This method will be useful for clinical applications involving the use of canine bone marrow-derived CD34^+ cells.
- 社団法人日本獣医学会の論文
- 2008-11-25
著者
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TSUJIMOTO Hajime
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The U
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FUJINO Yasuhito
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The U
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OHNO Koichi
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The U
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Tsujimoto Hajime
Department Of Veterinary Internal Medicine Graduate School Of Agricultural And Life Sciences The Uni
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MATSUURA SHINOBU
Department of Urology, Akita University School of Medicine
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Ohno Koichi
Department Of Veterinary Internal Medicine Graduate School Of Agricultural And Life Sciences The Uni
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Fujino Yasuhito
Department Of Veterinary Internal Medicine Graduate School Of Agricultural And Life Sciences The Uni
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IDE Kaori
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Science, The Un
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Matsuura Shinobu
Department Of Veterinary Internal Medicine Graduate School Of Agricultural And Life Sciences The Uni
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Matsuura Shinobu
Department Of Chemical Engineering Faculty Of Engineering Tokyo University
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Ohno Koichi
Department Of Veterinary Internal Medicine The University Of Tokyo
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Ohno Koichi
Department Of Chemical Engineering Faculty Of Engineering Niigata University
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Fujino Yasuhito
Univ. Tokyo Tokyo Jpn
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Ide Kaori
Department Of Veterinary Internal Medicine Graduate School Of Agricultural And Life Science The Univ
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Tsujimoto Hajime
Univ. Tokyo Tokyo Jpn
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Tsujimoto Hajime
Department Of Internal Medicine And Veterinary Hospital Division Of Veterinary Medicine The Universi
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