Temporal Dynamic Changes of Phenotypic Expression of Peripheral CD4 Cells during Environmental Allergen Challenge in an Experimental Model of Canine Atopic Dermatitis: A Pilot Study
スポンサーリンク
概要
- 論文の詳細を見る
The immunopathology behind atopic dermatitis (AD) involves a myriad of inflammatory cells and their mediators. Thymus and activation-regulated chemokine (TARC) plays a significant role in the inflammatory phase by recruiting CCR4+ TH2 cells. In addition, CD25+ activated T cells further propagate the allergic response after sensitization by producing cytokines. The purpose of this pilot study was to evaluate how exposure to a common allergen (house dust mite, HDM) would affect the proportions of circulating CD4+CCR4+ TH2 cells and CD4+CD25+activated T cells in an experimental model of AD using high-IgE Beagles. In this experimental model, previously sensitized Beagles develop lesions and pruritus upon allergen challenge consisting of 3-day environmental exposure, 3 hours/day. Blood samples were obtained before, during, and after the end of challenge (days 0, 2, 4, and 17). Clinical signs were evaluated and scored at the same time points. Peripheral blood mononuclear cells (PBMCs) were isolated and used for flow cytometry to identify proportions of CD4+cells positive for either CCR4 or CD25 receptors. Both CD4+cell types (CD25+ and CCR4+) peaked at day 17, when clinical signs had resolved. It is proposed that the increase of circulating CD4+CCR4+ and CD4+CD25+cells most likely demonstrates the sensitization status of atopic individuals after environmental allergen challenge. Understanding of these two cell types could prompt additional research concerning therapeutic drugs for AD.
著者
-
Maeda Sadatoshi
Department Of Veterinary Internal Medicine Graduate School Of Agricultural And Life Sciences The Uni
-
MARSELLA Rosanna
Blaunche Saunders Dermatology Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida
-
SIMPSON Andrew
Blaunche Saunders Dermatology Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida
関連論文
- Immunological Findings in 3 Dogs Clinically Diagnosed with Allergic Rhinitis
- Computer Tomography Diagnosis of Meningoencephalocele in a Calf
- Molecular Cloning of Canine Protease-Activated Receptor-2 and its Expression in Normal Dog Tissues and Atopic Skin Lesions
- Phenotypic Analysis for a Cell Line of Canine Epidermal Keratinocytes(Internal Medicine)
- Suitable Reference Genes for Quantitative Real-Time RT-PCR in Total RNA Extracted from Canine Whole Blood Using the PAXgeneTM System
- Temporal Dynamic Changes of Phenotypic Expression of Peripheral CD4 Cells during Environmental Allergen Challenge in an Experimental Model of Canine Atopic Dermatitis: A Pilot Study
- Production of GM-CSF Mediated by Cysteine Protease of Der f in Canine Keratinocytes
- Measurement for Canine IgE Using Canine Recombinant High Affinity IgE Receptor α Chain (FcεRIα)
- Magnetic Resonance Imaging Diagnosis of Dandy-Walker-Like Syndrome in a Wire-Haired Miniature Dachshund
- Insulin and Glucagon Secretory Patterns during Propionate and Arginine Tolerance Tests in Japanese Black Cattle with Growth Retardation
- Expression Analysis of CCL27 and CCL28 mRNA in Lesional and Non-Lesional Skin of Dogs with Atopic Dermatitis