Expression Analysis of CCL27 and CCL28 mRNA in Lesional and Non-Lesional Skin of Dogs with Atopic Dermatitis
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概要
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Chemokines are important regulators of the selective recruitment of inflammatory cells into sites of allergic inflammation. Since canine atopic dermatitis (AD) shares many clinical features of human AD, patterns of chemokine production in dogs may also be similar with those in humans. The aim of this study was to examine mRNA expression of CCL27 and CCL28 in lesional skin of dogs with AD to demonstrate similarity of chemokine production with human counterparts. RNA was extracted from skin biopsy specimens of 12 dogs with AD. The mRNA expression of CC chemokines (CCL4, CCL19, CCL20, CCL21, CCL24, CCL27 and CCL28) was analyzed by quantitative real-time PCR and was compared between lesional and non-lesional skin. Seven types of chemokines examined were constitutively expressed in both lesional and non-lesional skin. It was found that mRNA expression levels of CCL27 and CCL28 among the chemokines were significantly different between lesional and non-lesional skin (P<0.05). Expression level of CCL27 mRNA in lesional skin was significantly lower than that in non-lesional skin. On the other hand, CCL28 mRNA expression in lesional skin was found to be higher than that in non-lesional skin. These results suggest that CCL28 but not CCL27 may play important roles in immunopathogenesis of canine AD, indicating that experimental canine study may provide additional information that can be extrapolated to human AD.
- 公益社団法人 日本獣医学会の論文
著者
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Kitagawa Hitoshi
Department Of Electronic Science And Engineering Kyoto University
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FUKATA Tsuneo
Department of Veterinary Science, College of Agriculture
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Kawakami Tetsuji
Department Of Dermatology Faculty Of Medicine Tottori University
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Maeda Sadatoshi
Department Of Veterinary Internal Medicine Graduate School Of Agricultural And Life Sciences The Uni
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Tsuchida Hiromi
Department Of Veterinary Internal Medicine Faculty Of Applied Biological Sciences Gifu University
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Ohba Yasunori
Department of Materials Science and Engineering, Faculty of Engineering, Yamagata University
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SHIBATA Sanae
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
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OHBA Yasunori
Department of Veterinary Internal Medicine, Faculty of Applied Biological Sciences, Gifu University
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TSUKUI Toshihiro
Animal Life Science Laboratory, Nippon Zenyaku Kogyo Co., Ltd.
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FUKATA Tsuneo
Department of Veterinary Internal Medicine, Faculty of Applied Biological Sciences, Gifu University
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KAWAKAMI Tetsuji
Department of Veterinary Internal Medicine, Faculty of Applied Biological Sciences, Gifu University
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