Suitable Reference Genes for Quantitative Real-Time RT-PCR in Total RNA Extracted from Canine Whole Blood Using the PAXgeneTM System
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概要
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Since blood cells produce various soluble factors like cytokines or chemokines, gene expression analysis in whole blood could be important to investigate disease pathogenesis. In gene expression analysis with quantitative real-time RT-PCR, accurate determination of relative mRNA transcription levels requires appropriate reference genes. To identify the optimal reference gene in canine whole blood, we compared transcription levels of twelve candidate reference genes in total RNA extracted using the PAXgene system. The stability of the reference gene was evaluated by three different statistical programs, GeNorm, Normfinder and Bestkeeper. The results indicated that SDHA, CG14980 and TBP were the most stably expressed genes, which can be used as optimal reference genes for gene expression analysis in canine whole blood.
著者
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Maeda Sadatoshi
Department Of Veterinary Internal Medicine Graduate School Of Agricultural And Life Sciences The Uni
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KAMISHINA Hiroaki
Department of Veterinary Clinical Medicine, Faculty of Agriculture, Iwate University
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KIMURA Tsuyoshi
The United Graduate School of Veterinary Sciences, Gifu University
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KONDO Naho
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
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CHIMURA Naoki
The United Graduate School of Veterinary Sciences, Gifu University
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SHIBATA Sanae
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
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KAMISHINA Hiroaki
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
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MORI Takashi
Department of Applied Physics, Faculty of Engineering, Nagoya University
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HOSHINO Yuki
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
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MORI Takashi
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
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HOSHINO Yuki
Department of Veterinary Clinical Oncology, Gifu University
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KAMISHINA Hiroaki
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida
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