Mechanical Dechorionation of Fertilized Eggs for Experimental Embryology in the Medaka
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概要
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Naked developing zygotes of the medaka (Oryzias latipes) were easily obtained by cutting off part of the chorion of the vegetal pole area with scissors. A key point for this technique was the use of just-ovulated eggs before hardening of the chorion. When unfertilized eggs were inseminated after partial dechorionation, one spermatozoon penetrated and triggered the exocytosis of cortical alveoli at the animal pole. In these eggs, supernumerary spermatozoa also penetrated and transformed into pronuclei in the cortical cytoplasm of the vegetal pole region where the chorion was absent. The polyspermic egg proper spontaneously exited the chorion into sterile saline through the opening made by the cut. Most of these naked eggs developed normally, since the male pronuclei in the vegetal pole region did not contribute to syngamy. Dechorionated eggs with no surplus spermatozoa were obtained by inseminating eggs, rinsing them briefly in 10^<-3>% SDS to kill sperm in the medium, and removing the vegetal pole region of the chorion when the fertilized egg had begun to undergo cortical exocytosis at the animal pole ; such eggs also became spontaneously naked in saline. Denuded embryos developed normally to fry in sterile saline supplemented with 4 μg/ml gentamicin and 6% polyethylene glycol (M_r 1,540). Surgically operated embryos also developed to fry in the same medium. The present convenient technique for dechorionation and incubation of denuded eggs seems to be suitable for experimental embryology in the medaka.
- 社団法人日本動物学会の論文
- 1993-12-15
著者
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Iwamatsu Takashi
Department Of Biology Aichi University Of Education
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Fluck A.
Department Of Biology Franklin And Marshall College
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Fluck Richard
Department Of Biology Franklin And Marshall College
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Mori Takashi
Department Of Biochemistry And Cell Biology Institute Of Development And Aging Sciences Graduate Sch
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Mori Takashi
Department Of Biology Aichi University Of Education
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MORI Takashi
Department of Applied Physics, Faculty of Engineering, Nagoya University
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