Sex Reversal in the Medaka Oryzias latipes by Brief Exposure of Early Embryos to Estradiol-17β(Reproductive Biology)
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概要
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To induce sex reversal of male to female, freshly-fertilized eggs of the S-rR strain medaka (Oryzias laptipes) were immersed in saline containing estradiol-17β (E_2) in different concentrations for vaious durations until hatching. Results of the present experiment showed that the immersion duration in 1 μg/ml E_2 to introduce 100% reversal of sex differentiation in the genotypic males was enough only for one day (24hr) post-fertilization (dpf) and that treatment with E_2 for 1dpf resulted in a dose-dependent manner with the maximum sex reversal of 100% at 1 μg/ml. To ascertain early developmental periods efficacious for including sex reversal, additional brief immersion treatments of eggs with E_2 were further performed individually for four different early developmental period (Stages 4-9, 10-12, 13-15 and 16-18) within 1 dpf. As a result, induction of sex reversal was observed in all these short immersion periods without any restricted efficacy. Between both experimental and control groups treated with or without E_2 for 1 dpf, differences in the number of germ cells in a gonad were compared in newly-hatched fry. It was found that gonads of the genotypic males (XY) treated with E_2 revealed the female type which contained many germ cells with much dividing activity. These data suggest that a possible switch mechanism that exogenous E_2 could trigger to change the genetic cascades involved in sex determination upon fertilization exists in early developmental stages.
- 2005-10-25
著者
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Iwamatsu Takashi
Department Of Biology Aichi University Of Education
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Iwamatsu Takashi
Aichi Univ. Education Aichi Jpn
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Kobayashi Hirokuni
Department Of Biology Asahi University School Of Dentistry
関連論文
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