In Vivo Fertilization and Development of Medaka Eggs Initiated by Artificial Insemination(Reproductive Biology)
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概要
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Fertilization and development in the ovarian cavity of oviparous fish, Oryzias latipes, were examined using the S-rR strain in which the sex genotype can be easily distinguished by the body color of the fish. Mature eggs were fertilized within the ovarian cavity after a sperm suspension was artificially introduced with a small bore-pipette through the urinogenital opening. Three batches of eggs ovulated within 48 hrs were fertilized and began to develop in the ovarian cavity, while eggs ovulated 72 hrs post-insemination (PI) were no longer fertilized. These observations indicate that ovulation occurs irrespective of the existence of developing embryos within the ovarian cavity. All embryos developing in the ovarian cavity were, however, retarded and ceased development before the stage of initiation of blood circulation at room temperature. These embryos developed normally and hatched after they were transferred from the ovarian cavity into regular saline 48 or 72 hrs PI. When these individuals matured sexually, their sex differentiation was found to be normal, and sex reversal was not observed.
- 2005-01-25
著者
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Iwamatsu Takashi
Department Of Biology Aichi University Of Education
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Kobayashi Hirokuni
Department Of Biology Asahi University School Of Dentistry
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Sato Masahiro
World's Medaka Aquarium, Nagoya Higashiyama Zoo
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Sato Masahiro
World's Medaka Aquarium Nagoya Higashiyama Zoo
関連論文
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- Regional Differences in Granulosa Cells of Preovulatory Medaka Follicles
- Distribution and phylogeny of rice fish and their relatives belonging to the suborder Adrianichthyoidei in Sulawesi, Indonesia
- Sex reversal in medaka treated in vitro with 17α-methyldihydrotestosterone during oocyte maturation
- Experimental Hybridization among Oryzias Species. II. Karyogamy and Abnormality of Chromosome Separation in the Cleavage of Interspecific Hybrids between Oryzias latipes and O. javanicus
- Studies on fertilization in the teleost IV. Effects of aphidicolin and camptothecin on chromosome formation in fertilized medaka eggs
- Studies on fertilization of the teleost. II. Nuclear behavior and changes in histone H1 kinase
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- Changes in chorion proteins induced by the exudate released from the egg cortex at the time of fertilization in the teleost, Oryzias latipes
- SEX REVERSAL IN THE MEDAKA TREATED IN VITRO 17α-METHYLDIHYDROTESTOSTERONE DURING OOCYTE MATURATION(Endocrinology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- SEX REVERSAL IN THE MEDAKA ORYZIAS LATIPES BY BRIEF IMMERSION TREATMENT OF FERTILIZED EGGS TO NONAROMATIZABLE ANDROGEN(Endocrinology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- In Vivo Fertilization and Development of Medaka Eggs Initiated by Artificial Insemination(Reproductive Biology)
- SEX REVERSAL IN THE MEDAKA (ORYZIAS LATIPES) BY A SINGLE BRIEF EXPOSURE OF FERTILIZED EGGS TO ESTRADIOL-17β(Endocrinology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- RAPID COMMUNICATION : Sperm Behavior in the Micropyle of the Medaka Egg
- Effect of Micropylar Morphology and Size on Rapid Sperm Entry into the Eggs of the Medaka
- Oogenesis in the Medaka Oryzias latipes : Stages of Oocyte Development : Developmental Biology
- Inorganic contents of the medaka egg before and after cortical reaction
- Changes in the chorion and sperm entry into the micropyle during fertilization in the teleostean fish, Oryzias latipes
- Oil droplets and yolk spheres during development of Medaka embryos
- Egg Activation
- Stages of Normal Development in the Medaka Oryzias latipes
- Scientist, H. Uwa passed away at 53 : a taxonomist of the medaka
- Evidence for Involvement of the Exudate Released from the Egg Cortex in the Change in Chorion Proteins at the Time of Egg Activation in Oryzias latipes
- Dynamic Growth of Oocytes of the Medaka, Oryzias latipes : I. A Relationship between Establishment of the Animal-Vegetal Axis of the Oocyte and Its Surrounding Granulosa Cells
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- On a Relationship between Oocyte and Follicle Cells around the Time of Ovulation in the Medaka,Oryzias latipes
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- Studies on fertilization in the teleost. I. Dynamic responses of fertilized medaka eggs
- Karyotype and banding analyses of Oryzias celebensis (Oryziatidae, Pisces) in cultured cells.
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