魚卵毒の検索〔英文〕

概要

論文の詳細を見る
A screening test for ichtyootoxin, the toxin of fish roe, was performed on 23 species including the fishes suspected to be ichthyootoxic. The roes of two fishes, the killifish Fundulus heteroclitus and the blenny Stichaeus nozawai, were found to be toxic. A toxic lipid was extracted with chloroform-methanol (2:1) from killifish roe. Its chromatographic behavior on silicic acid columns was similar to that of dinogunellin, the Japanese northern blenny roe toxin. However, it did not exhibit the u.v. absorption maximum characteristic of dinogunellin. In addition, the 10% saline extract of the roe was nontoxic, indicating that the toxin does not occur as a lipoprotein in the roe. A toxic component of blenny roe was extracted with chloroform-methanol, and was successfully purified using the procedure for dinogunellin. The purified toxin was indistinguishable from dinogunellin both in various qualitative tests and in chromatographic behavior. The saline extract was also toxic and afforded a toxic phospholipid indistinguishable from dinogunellin in TLC when extracted with chloroform-methanol (2:1). These results strongly indicate that dinogunellin and its closely related substance(s) are distributed widely in the roes of marine ichthyootoxic fishes.