リパ-ゼを用いて合成した高度不飽和脂肪酸含有油脂の分子種

概要

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Enzymatic glyceride synthesis and acid interchange using icosapentaenoic acid (EPA), docosahexaenoic acid (DHA), γ-linolenic acid (GLA), and EPA and DHA concentrated saponified fatty acid mixture obtained from sardine oil were carried out through the use of four kinds of microbial lipases. Lipase TOYO (Chromobacterium viscosum) was the most effective enzyme for glyceride synthesis as well as acid interchange of triglyceride (TG) rich in polyunsaturated fatty acids. There was a general tendency, except with Lipase TOYO, that DHA was difficult to be incorporated into a glycerol molecule compared to the other fatty acids. Fatty acid composition of TG synthesized from a highly concentrated fatty acid mixture (EPA+DHA: 68.8%) by the use of Lipase TOYO and Lipase OF (Candida cylindracea), directly reflected that of the substrate fatty acid flixture (i.e. EPA+DHA was more than 65%). And the TG molecular species having combination of only EPA and DHA such as (EPA, EPA, EPA), (EPA, EPA, DHA), (EPA, DHA, DHA), and (DHA, DHA, DHA) were dominant accounting for the half of the obtained TG.
日本水産學會の論文