工業用ホスホリパーゼA2による高度不飽和脂肪酸含有リン脂質の合成

概要

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乳化剤として市販されているリゾ型リン脂質より調製したLPC，ならびにイワシ油をケン化して調製したHUFAを基質として用い，ブタ膵臓由来ホスホリパーゼA2触媒によるHUFA含有PCの合成を行った．合成されたPCの脂肪酸の濃度は，基質として用いた脂肪酸の濃度に影響され，2位の位置に選択的に導入されることが明らかになった．Phospholipids consist of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were synthesized by use of an industrial pancreatic phospholipase A2. Lysophosphatidylcholine (LPC) isolated from a commercial emulsifier, mixed fatty acids obtained from sardine oil, the concentrated sardine oil (EPA+DHA>60%), and 99.4% EPA were used as substrates. Optimum water content was found to 13.4%/system, and optimum ratio of LPC to mixed free fatty acids was 11:18 in weight base. When the substrate contained free EPA+DHA over 68%, the obtained phosphatidylcholine (PC) contained more than 31% of EPA+DHA. And when the substrate was 99.4% EPA, the obtained PC contained more than 45% of EPA, indicating that more than 90% of EPA was incorporated into sn-2 position of the PC molecule.
日本食品工業学会の論文