沖繩毒魚肝臓中の水溶性の毒,シガテリンの分離

概要

論文の詳細を見る
Ciguaterin, an emetic factor, was extracted from the liver of the red snapper Lutjanus bohar from the Ryukyu Islands and purified by column chromatography using ion-exchange resins, acidic alumina, silicic acid, and Sephadex G-10 successively. The final product evoked vomiting in cats within 30min at a dose as low as 3μg/kg. It was however still contaminated by a few ninhydrin-positive substances, as shown by thin-layer chromato-graphy. The purified emetic was positive to ninhydrin, peptide and Pauly reagents, but negative to Dragendorff and sugar reagents. It showed only a slight shoulder near 330nm in the ultraviolet region. On acid hydrolysis, it afforded 14 amino acids, of which lysine, glutamic and aspartic acids were prominent. Total amino acid residues accounted for 40 per cent of the solid. These results suggest that ciguaterin possesses a peptide moiety.
公益社団法人日本水産学会の論文